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Plasmid#172169PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationInserted amber codon immediately after initiator …PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB061i2
Plasmid#79229PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-HA4 (Y87A); PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP_LD 0_TEV
Plasmid#58877PurposeMESA protease chain with Rapamycin FKBP ectodomain and no linker domainDepositorInsertMESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
UseAAVTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (TBE 1/2 - wt)
Plasmid#16595DepositorInsertc-myc promoter (TBE 1/2 wt) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pconst #4-RFP
Plasmid#81078Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23107Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-AMOT p130 delta linker
Plasmid#32825DepositorInsertAMOT p130 (AMOT Human)
UseTagsHAExpressionMammalianMutationE5D and amino acids 752-1076 deletedPromoterCMVAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-NHX1-HaloTagx2
Plasmid#177107PurposeExpresses Nhx1-HaloTagx2 in yeast cellsDepositorInsertNHX1 (NHX1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-AMOT p130 deltaEYLI
Plasmid#32826DepositorInsertAMOT p130 (AMOT Human)
UseTags2xHAExpressionMammalianMutationE5D, deletion of last 4 amino acids (EYLI)PromoterCMVAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV- CAG-h2BmRuby2FLAG
Plasmid#116872PurposeNuclear localized mRuby2FLAGDepositorInsertH2BmRuby2FLAG
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
miR-vector-RFP pCDNA
Plasmid#97143Purposefor expression of miRNA and TagRFPDepositorTypeEmpty backboneUseTagsTagRFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG TIF1gamma M (456-887)
Plasmid#15735DepositorInsertTIF1 gamma M (TRIM33 Human)
UseTagsFlagExpressionMammalianMutationMiddle region (amino acids 456-887).PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisA-rat-PTP1B
Plasmid#35987DepositorInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
UseTagsHis and Xpress Epitope TagExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsUPF2-siRNAres_I
Plasmid#146567PurposeMammalian Expression of HsUPF2-siRNAresDepositorInsertHsUPF2-siRNAres (UPF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Archon-EGFP
Plasmid#187979PurposeExpresses the fluorescent voltage indicator Archon under the GFAP promoterDepositorInsertArchon1-EGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SP6-hCas9-Ce-mRNA
Plasmid#47911PurposeIn vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.DepositorInserthCas9 with C. elegans 5' and 3' UTRs
UseCRISPRTagsExpressionMutationPromoterSP6Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
4XBS2WT-Luc
Plasmid#16593DepositorInsertp53 binding sites (TP53 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/plsA
Plasmid#73625PurposeProduces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tagDepositorInsertadenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only