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Plasmid#120985PurposeMoClo golden gate assembly CD part for LasR-LSTPLVMI (Adam Meyer's improved LasR activator, selected for dynamic range and low cross-sensitivity with methods from https://doi.org/10.1101/285866).DepositorInsertLasR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C74m
Plasmid#120986PurposeMoClo golden gate assembly CD part for LuxRQ-FMY (Adam Meyer's improved LuxR activator from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertLuxR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C75m
Plasmid#120987PurposeMoClo golden gate assembly CD part for NahRQ-VHAC (Adam Meyer's improved NahR activator from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertNahR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P66m
Plasmid#120954PurposeMoClo golden gate assembly AB part for pRpa-AM (Adam Meyer's improved pRPA promoter from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertpRpa Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P12m
Plasmid#120938PurposeMoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pLasTetO
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P15m
Plasmid#120939PurposeMoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinTetO
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P33m
Plasmid#120944PurposeMoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP11 from Mutalik library (medium)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P34m
Plasmid#120945PurposeMoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP13 from Mutalik library (weak)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47732:FCP:ShBle
Plasmid#85985PurposeExpresses ShBle with FCP promoter/terminator for F. cylindrus transformation.DepositorInsertShBle
UseGolden gate assemblyTagsExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIE096 CMV Bxb1
Plasmid#139252PurposeEncodes a Bxb1 recombinase for Landing Pad integration.DepositorInsertBxb1 Recombinase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1019 TRE3GV DsRedExpress2 in TU2
Plasmid#139254PurposeTRE3GV promoter and DsRedExpress2 in TUPV2DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM467 TRE3GV EYFP in TU3
Plasmid#139255PurposeTRE3GV promoter and EYFP in TUPV3DepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PT7-alphaHL
Plasmid#227650PurposepT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.DepositorInsertalpha-hemolysin
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBD006
Plasmid#201306PurposeD16: Promoter of TraesCS5D02G320700 as Golden Gate compatible MoClo PROM partDepositorInsertD16: Promoter of TraesCS5D02G320700
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBD004
Plasmid#201304PurposeD14: Promoter of TraesCS3D02G368800 as Golden Gate compatible MoClo PROM partDepositorInsertD14: Promoter of TraesCS3D02G368800
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBD005
Plasmid#201305PurposeD15: Promoter of TraesCS3D02G517500 as Golden Gate compatible MoClo PROM partDepositorInsertD15: Promoter of TraesCS3D02G517500
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD1023 TRE3GV DsRedExpress2 in TU5
Plasmid#139257PurposeTRE3GV promoter and DsRedExpress2 in TUPV5DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
C77m
Plasmid#120989PurposeMoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAmpR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C78m
Plasmid#120990PurposeMoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertChlorR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyTagsExpressionMutationBsaI site removedPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only