We narrowed to 4,399 results for: BFP

1. pRCA594 - pBA904 Puro-T2A-BFP

   Plasmid

   #238170

   Purpose

   Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   Non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   mTagBFP2

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

   Plasmid

   #236743

   Purpose

   Constitutive expression of Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
   EF1a-hygro-WPRE
   

   Expression

   Mammalian

   Promoter

   CAG and EF1a

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

   Plasmid

   #236245

   Purpose

   Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Promoter

   U6 and human Synapsin1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

   Plasmid

   #223110

   Purpose

   pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpG-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

   Plasmid

   #223111

   Purpose

   pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. XZ076 (PB) CMV-mCCL20 (PuroR-BFP)

   Plasmid

   #233433

   Purpose

   PiggyBac donor plasmid, membrane-bound CCL20

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   CCL20-pre-mGRASP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-FLAG-HAND1-T2A-mTagBFP2

   Plasmid

   #223192

   Purpose

   Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   HAND1 (HAND1 Human)
   

   Use

   Promoterless entry vector for gateway cloning

   Tags

   FLAG and T2A-mTagBFP2

   Expression

   Bacterial

   Promoter

   None

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

   Plasmid

   #231354

   Purpose

   Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   BC(p1-10)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS3-rab-3P::AI::QF+hGR::SL2::tagBFP

   Plasmid

   #232607

   Purpose

   Pan-neuronal expression of chimeric protein 'QF+hGR' and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

   Depositor

   Andrew Leifer

   Article

   Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

   Insert

   QF+hGR
   

   Expression

   Worm

   Mutation

   N/A

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

    Plasmid

    #229797

    Purpose

    Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC-hU6-crBACH1-array_EF1a-BFP

    Plasmid

    #224787

    Purpose

    BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBACH1-1, crBACH1-2, crBACH1-3,
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC-hU6-crCoV-Mutiplex_EF1a-BFP

    Plasmid

    #224788

    Purpose

    SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crCoV20, crCoV21, crCoV24
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-IRES-mTagBFP2-pA (JDW 1361)

    Plasmid

    #224535

    Purpose

    A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    IRES-FLAG-NLS-mTagBFP2-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. L0-T12 GG2-ER-tagBFP-tUBQ10-GG3

    Plasmid

    #219695

    Purpose

    Level 0 to construct history-dependent target

    Depositor

    Jennifer Nemhauser

    Insert

    GG2-ER-tagBFP-tUBQ10-GG3
    

    Use

    Synthetic Biology

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

    Plasmid

    #219689

    Purpose

    Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2PsB (mtagBFP2, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Hyg-BFP-P2A-ppViperin

    Plasmid

    #217481

    Purpose

    Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

    Depositor

    Bertrand Collet

    Article

    Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

    Insert

    BFP-P2A-otPKRFL
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Pur-BFP-P2A-ppViperin

    Plasmid

    #217483

    Purpose

    Expression of Blue Fluorescent Protein - P2A - short length protein kinase R from chinook salmon

    Depositor

    Bertrand Collet

    Insert

    BFP-P2A-otPKRSL
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Zeo-BFP-P2A-ppViperin

    Plasmid

    #217482

    Purpose

    Expression of Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmon

    Depositor

    Bertrand Collet

    Insert

    BFP-P2A-otPKRML
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Zeo-BFP-P2A-otPKR-ML

    Plasmid

    #214365

    Purpose

    Expression of the Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmon

    Depositor

    Bertrand Collet

    Article

    Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

    Insert

    BFP-P2A-otPKRML
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Hyg-BFP-P2A-otPKR-ML

    Plasmid

    #214361

    Purpose

    Expression of the Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmon

    Depositor

    Bertrand Collet

    Insert

    BFP-P2A-otPKRML
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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