-
Plasmid#169550PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertcHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2304-Tier1-Tactb-PPGK-NLuc_TB
Plasmid#169549PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertTactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2303-Tier1-CoTC-PPGK-NLuc_TB
Plasmid#169548PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertCoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rosa26_gRNA
Plasmid#196138PurposegRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2DepositorInsertRosa26 gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-CreLite
Plasmid#131783PurposeTol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.DepositorInsertPhyBCreC, PIF6CreN, and mTagBFP2
UseCre/Lox and Synthetic Biology; Zebrafish tol2 tra…TagsmTagBFP2ExpressionMammalianMutationPromoterUbiAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNMSB96
Plasmid#199316PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, C. elegans codon optimized …Promoterflp-18Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2308-Tier1-pGL3-PPGK-NLuc_TB
Plasmid#169551PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertpGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNMSB84
Plasmid#199309PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…Promoterflp-18Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB99
Plasmid#199308PurposeMosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertmyo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…Promotermyo-3Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB104
Plasmid#199314PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, C. elegans codon optimized …Promoterflp-18Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF139
Plasmid#135924PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mTagBFP
UseTransposonTagsmTagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)
Viral Prep#100798-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
cfSYFP2-N
Plasmid#110631PurposeCys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.DepositorInsertcfSYFP2-N
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGH896
Plasmid#230840PurposeEf1a-DivA-BE-T2A-tagBFP-wPREDepositorInsertDivA-BE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH915
Plasmid#230843PurposeEf1a-BE3-T2A-tagBFP-wPREDepositorInsertBE3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH720
Plasmid#230845PurposeEf1a-ABE7.10-T2A-tagBFP-wPREDepositorInsertABE7.10
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH897
Plasmid#230841PurposeEf1a-nDivA-BE-T2A-tagBFP-wPREDepositorInsertnDivA-BE3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH905
Plasmid#230844PurposeEf1a-PmCDA1-BE3-T2A-tagBFP-wPREDepositorInsertPmCDA1-BE3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH483
Plasmid#230835PurposeEf1a-dCas9-T2A-tagBFP-wPREDepositorInsertdCas9
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH484
Plasmid#230838PurposeEf1a-AID*-dCas9-T2A-tagBFP-wPREDepositorInsertAID*-dCas9
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only