Skip to main content
Addgene
Showing: 1661 - 1680 of 1977 results

These results may also be relevant.

  2. pOCC177

    Plasmid
    #118886
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and SNAP tag…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118886-plasmid-map-sequence-id-232379

    Enlarge

  3. pOCC194

    Plasmid
    #118882
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    SNAP tag, cleavable with TEV protease, HIS6, clea…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118882-plasmid-map-sequence-id-232359

    Enlarge

  4. pOCC210

    Plasmid
    #118896
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and SNAP tag, cle…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118896-plasmid-map-sequence-id-232311

    Enlarge

  5. pOCC231

    Plasmid
    #118885
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and mCitrine…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118885-plasmid-map-sequence-id-232309

    Enlarge

  6. pOPTXGARE

    Plasmid
    #68542
    Purpose
    cyclic nucleotide reporter for bacteria or plant cells
    Depositor
    Helen Irving
    Article
    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1
    Insert
    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    Use
    Tags
    luciferase
    Expression
    Bacterial and Plant
    Mutation
    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…
    Promoter
    OPTX promoter with GARE
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68542-plasmid-map-sequence-id-121994

    Enlarge

  7. pUDP010

    Plasmid
    #101166
    Purpose
    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101166-plasmid-map-sequence-id-199274

    Enlarge

  8. pDONR P4-P1R-ECFP

    Plasmid
    #48352
    Purpose
    Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    ECFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a Kozak sequence. Does not contain a sto…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48352-plasmid-map-sequence-id-70571

    Enlarge

  10. PCUP1-GFP-Pho8-406

    Plasmid
    #207006
    Purpose
    Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    PHO8
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207006-plasmid-map-sequence-id-422251

    Enlarge

  11. PCUP1-GFP-Osh1-406

    Plasmid
    #207007
    Purpose
    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    OSH1
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207007-plasmid-map-sequence-id-422254

    Enlarge

  17. pMM7

    Plasmid
    #201213
    Purpose
    Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.
    Depositor
    Alex Nielsen
    Article
    Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1
    Inserts
    sfGFP
    Spo0A right flank
    Spo0A left flank
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    F11V, T13P, N39D, A179A
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201213-plasmid-map-sequence-id-405310

    Enlarge

  18. eSrtA-MT3

    Plasmid
    #200305
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT3 (MT3 Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200305-plasmid-map-sequence-id-394866

    Enlarge

  19. pYPQ230-RRVQ

    Plasmid
    #200188
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRVQ
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V, E795Q
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200188-plasmid-map-sequence-id-396764

    Enlarge

  20. pYPQ230-D156R

    Plasmid
    #188542
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-D156R
    Use
    CRISPR; Gateway compatible lbcas12a-d156r entry c…
    Tags
    NLS
    Expression
    Plant
    Mutation
    D156R
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188542-plasmid-map-sequence-id-369465

    Enlarge

Showing: 1661 - 1680 of 1977 results