Skip to main content
Addgene
Showing: 1661 - 1680 of 6795 results

  1. pTEV5_ RpMatB_1

    Plasmid
    #78205
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7
    Insert
    matB (His6 C106A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. pICSL50016

    Plasmid
    #50315
    Depositor
    Nicola Patron
    Article
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Insert
    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    Use
    Plant expression
    Tags
    Expression
    Mutation
    Codon-optimised for A. thaliana. BsaI/BbsI sites …
    Promoter
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50315-plasmid-map-sequence-id-75072

    Enlarge

  7. pET:GACG:GFP

    Plasmid
    #60735
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60735-plasmid-map-sequence-id-97721

    Enlarge

  8. pICSL70002

    Plasmid
    #50333
    Depositor
    Nicola Patron
    Article
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Insert
    Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
    Use
    Plant expression
    Tags
    Expression
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Promoter
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50333-plasmid-map-sequence-id-194302

    Enlarge

  13. pUASTattb-cAMPr Y205A, R626A

    Plasmid
    #84932
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R626A
    Use
    Tags
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R626A
    Promoter
    5X UAS
    Available since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84932-plasmid-map-sequence-id-180302

    Enlarge

  14. pUASTattb-cAMPr Y205A, R739A

    Plasmid
    #84933
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R739A
    Use
    Tags
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R739A
    Promoter
    5X UAS
    Available since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84933-plasmid-map-sequence-id-180303

    Enlarge

  18. Nabi2.244

    Plasmid
    #73756
    Purpose
    Nabi2.244 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.244
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73756-plasmid-map-sequence-id-136688

    Enlarge

Showing: 1661 - 1680 of 6795 results