Addgene

We narrowed to 4,181 results for: 027

Showing: 1661 - 1680 of 4181 results

Results per page:

Sort results by:

pD649-HAsp-ROBO1-COMP5AP-AviTag-9xHis

Plasmid

#157562

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ROBO1 (ROBO1 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis

Plasmid

#157396

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HLA-E (HLA-E Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-VSTM2L-Fc(DAPA)-AviTag-6xHis

Plasmid

#156582

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSTM2L (VSTM2L Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-VCAM1-COMP5AP-AviTag-9xHis

Plasmid

#157506

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VCAM1 (VCAM1 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-FullLength-TET1-CXXC/LLAA

Plasmid

#124399

Purpose

Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

CXXC zinc finger deletion (aa 585-624) and L897A/…

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-ZNF655

Plasmid

#88578

Purpose

Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF655 (ZNF655 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-Nr4a1sgRNA1

Plasmid

#160945

Purpose

Guide RNA 1 to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 gRNA 1 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3-SP-6xHis-KIR2DL1(FL)-FLAG

Plasmid

#157625

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL1 (KIR2DL1 Human)

Use

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-TNFRSF6B-COMP5AP-AviTag-9xHis

Plasmid

#157277

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF6B (TNFRSF6B Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SIGLEC1(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157610

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC1 (SIGLEC1 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p029 End-1::EnR

Plasmid

#11029

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Dominant inhibitory GATA-binding protein End1::En…

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3-SP-6xHis-KIR2DL4(FL)-FLAG

Plasmid

#157624

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL4 (KIR2DL4 Human)

Use

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GSK3B gRNA (BRDN0001146763)

Plasmid

#77283

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3B (GSK3B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-LAIR2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156520

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LAIR2 (LAIR2 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-Grpr1

Plasmid

#175176

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grpr

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

sgRNA-Grpr

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-crRNA(SOD1)

Plasmid

#109321

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (SOD1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Dscaml1-AP-His

Plasmid

#71946

Purpose

Expresses the extracellular region of the Dscaml1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscaml1 (Dscaml1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-crRNA(TBK1)

Plasmid

#109322

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TBK1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HKDC1 gRNA (BRDN0001148616)

Plasmid

#76827

Purpose

3rd generation lentiviral gRNA plasmid targeting human HKDC1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HKDC1 (HKDC1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge