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Addgene
Showing: 1641 - 1660 of 4623 results

  1. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Use
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

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  2. pHN872

    Plasmid
    #178830
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Use
    Tags
    GST, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    178830-plasmid-map-sequence-id-351931

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  3. pHN1002

    Plasmid
    #178831
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Use
    Tags
    His8, 3C
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    178831-plasmid-map-sequence-id-351933

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  6. HiFi dFE

    Plasmid
    #190142
    Purpose
    Expresses HiFiCas9-T4pol (HiFi dFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    HiFi Cas9-T4pol (HiFi dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    SpCas9 R691A mutant, Codon Optimized for mammalia…
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    190142-plasmid-map-sequence-id-374983

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  7. LZ3 iFE

    Plasmid
    #190143
    Purpose
    Expresses LZ3Cas9-POLB (LZ3 iFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    LZ3Cas9-POLB (LZ3_iFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    190143-plasmid-map-sequence-id-374873

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  8. LZ3 dFE

    Plasmid
    #190144
    Purpose
    Expresses LZ3Cas9-T4pol (LZ3 dFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    LZ3Cas9-T4pol (LZ3 dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    190144-plasmid-map-sequence-id-376723

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  9. pISV-CRISPR/Cas9PGmUbi

    Plasmid
    #209189
    Purpose
    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
    Depositor
    Christian Staehelin
    Inserts
    Cas9
    DsRed(deltaB)
    Use
    Tags
    NLS
    Expression
    Plant
    Mutation
    missing BsaI site and plant-codon optimized with …
    Promoter
    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…
    Availability
    Academic Institutions and Nonprofits only
    209189-plasmid-map-sequence-id-419145

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  13. AAV1-DIO-eGFP-pvRPL10a

    Plasmid
    #202542
    Purpose
    Cr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.
    Depositor
    Zoe Donaldson
    Article
    Sadino et al Elife. 2023 Feb 28;12:e80517. doi: 10.75
    Insert
    RPL10a subunit of the ribosome
    Use
    AAV and Cre/Lox
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    optimized for prairie vole DNA sequence and optim…
    Promoter
    human synapsin
    Availability
    Academic Institutions and Nonprofits only
    202542-plasmid-map-sequence-id-405350

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  14. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

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  17. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid
    #197506
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
    197506-plasmid-map-sequence-id-386645

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Showing: 1641 - 1660 of 4623 results