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Plasmid#161876PurposeIn vitro transcription of SpCas9DepositorInserthuman codon optimized SpCas9
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/DdmEGFP
Plasmid#154304Purposecloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideumDepositorInsertmEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pKG113
Plasmid#63073PurposeRetroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)DepositorInsertCENP-T (CENPT Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationE. coli codon optimized. G217R mutation compared …PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHN873
Plasmid#178829PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsMBP, His8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIK86
Plasmid#47933PurposeC. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.DepositorInsertCas9
UseCRISPR and UnspecifiedTagsExpressionMutationPromoterT7Available sinceOct. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTurquoise2
Plasmid#118453PurposeOverexpression of yeast codon-optimized mTq2DepositorInsertymTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-QSco
Plasmid#46106DepositorInsertQSco
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133568
Plasmid#152340PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDD372
Plasmid#91824PurposeCodon-optimized GFP donor for the SapTrap cloning systemDepositorInsertGFP-C1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD377
Plasmid#91823PurposeCodon-optimized mTurquoise2 donor for the SapTrap cloning systemDepositorInsertmTurquoise2-C1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LZ3 iFE
Plasmid#190143PurposeExpresses LZ3Cas9-POLB (LZ3 iFE)DepositorInsertLZ3Cas9-POLB (LZ3_iFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133731
Plasmid#152742PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4.26-SS-358
Plasmid#68790PurposeZF9 Op x6 -- NTRDepositorInsertsZF9 Op x6
Nitroreductase
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 dFE
Plasmid#190137PurposeExpresses SpCas9-T4pol (dFE)DepositorInsertSpCas9-T4pol (dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/DdmRuby3
Plasmid#154305Purposecloned with the codon-optimized mRuby3 gene for expression in Dictyostelium discoideumDepositorInsertmRuby3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
HSD11B1
Plasmid#39155PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHSD11B1 (HSD11B1 Human)
UseTagsHis TevExpressionBacterialMutationT220I; codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133256
Plasmid#152133PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133586
Plasmid#152328PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133862
Plasmid#152487PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits