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Plasmid#147307PurposeBacterial Expression of HsNot11DepositorInsertHsNot11 (CNOT11 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPasilla_B
Plasmid#145943PurposeInsect Expression of DmPasillaDepositorInsertDmPasilla (ps Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAUB_A
Plasmid#145851PurposeInsect Expression of DmAUBDepositorInsertDmAUB (aub Fly)
UseTagsExpressionInsectMutationTwo silent mutations compared to the sequence giv…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP2_457-791_A
Plasmid#145854PurposeInsect Expression of DmDCP2_457-791DepositorInsertDmDCP2_457-791 (DCP2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalphaKanR
Plasmid#160230PurposeLike pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorInsertU6-BbsI-chiRNA
UseCRISPRTagsExpressionInsectMutationPromoterDm-snRNA:U6:96AbAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAct:dCas9
Plasmid#78903PurposeExpresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertdCas9
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pNos-PE2-attB
Plasmid#149549PurposeExpression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.DepositorInsertPE2
UseCRISPRTagsExpressionInsectMutationPromoternanos (nos)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159672PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertβTub-NLS-hSpCas9-T2A-GFP
UseTagsT2A-GFPExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182
Plasmid#22419DepositorInsertgawky, variant A (gw Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRIV-Gal4DBD:2xnMagHigh1
Plasmid#182031PurposeDonor plasmid for conversion of KO attP Drosophila lines into ShineGal4 driversDepositorInsertGal4DBD2xHighMag1-p103'UTR
UseDonor plasmidTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159671PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertExuL-NLS-hSpCas9-T2A-GFP
UseTagsT2A-GFPExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-lambdaN-HA-DmTral
Plasmid#21721DepositorInsertTrailer hitch (tral Fly)
UseDrosophila expressionTagslambdaN-HAExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP2-V5His6
Plasmid#22409DepositorInsertdecapping protein 2, variant A (DCP2 Fly)
UseDrosophila expressionTagsV5-His6 and lambdaN-HAExpressionMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
CJ40 (let-7 seed mutant perfect site)
Plasmid#17773DepositorInsertlet-7 seed mutant perfect site (let-7 Fly)
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationseed mutantPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab18 DN
Plasmid#58722Purposeexpression of drosophila YFP Rab18 dominant negative mutantDepositorInsertRab18 (Rab18 Fly)
UseTagsYFPExpressionInsectMutationS19NPromoterGAL4Available sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only