Addgene

We narrowed to 2,510 results for: fas

Showing: 1621 - 1640 of 2510 results

Results per page:

Sort results by:

CVS-N2c-EGFP-ChIEF

Plasmid

#172371

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

EGFP + ChIEF

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-nl.EGFP-FlpO

Plasmid

#172379

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + FlpO

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP_cPPVp

Plasmid

#118967

Purpose

Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cPPVp in fusion with FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FRB_nPPVp

Plasmid

#118966

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with FRB

Use

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-SypRFP

Plasmid

#172381

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

Synaptophysin1-RFP fusion protein

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
dsRedm-Rab21

Plasmid

#83425

Purpose

Full-length mouse Rab21 (wildtype)

Depositor

Johanna Ivaska

Article

Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

Insert

Rab21 (Rab21 Mouse)

Use

Tags

dsRed-monomer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_STf_LamB1

Plasmid

#181999

Purpose

CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf-LamB1

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGREAT18

Plasmid

#170906

Purpose

GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pAtUBQ10

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-EGFP-FlpO

Plasmid

#172373

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

EGFP + FlpO

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-jGCaMP8f

Plasmid

#172387

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

GCaMP8f

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CVS-N2c-nl.EGFP-tdTomato

Plasmid

#172382

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + tdTomato

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX2-FLAG-Smad4

Plasmid

#89834

Purpose

Express Smad4 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

SMAD4 (SMAD4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGREAT1

Plasmid

#170889

Purpose

GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pNOS

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSV human p55 alpha (HA tag)

Plasmid

#11498

Depositor

Ronald Kahn

Article

Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

Insert

p55 alpha (PIK3R1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pJ414-PCY1

Plasmid

#98150

Purpose

Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mScarlet-I-SYFP2

Plasmid

#189761

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-syn-fRCaMP1

Plasmid

#125245

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP1

Use

Tags

Expression

Mammalian

Mutation

W44Y

Promoter

synapsin

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-NEW-fRGECO2

Plasmid

#125248

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Use

Tags

NES signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
cycLuc_SbMVs

Plasmid

#119209

Purpose

Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with SbMVp cleavage site

Use

Luciferase

Tags

AU1 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge