Showing: 1621 - 1640 of 7113 results
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Plasmid#75029PurposetagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInserttagBFP2^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized tagBFP2ExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHH0103 WWTR1 WW domain
Plasmid#104261PurposeBacterial expression of WW domain from WWTR1DepositorInsertWWTR1 WW domain (WWTR1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pHH0103 STXBP4 WW domain
Plasmid#104265PurposeBacterial expression of WW domain from STXBP4DepositorInsertSTXBP4 WW domain (STXBP4 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pHH0103 PIN1L WW domain
Plasmid#104269PurposeBacterial expression of WW domain from PIN1LDepositorInsertPIN1L WW domain (PIN1P1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pHH0103 UTROPHIN WW domain
Plasmid#104275PurposeBacterial expression of WW domain from UTROPHINDepositorInsertUTROPHIN WW domain (UTRN Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pPICZaa-STE2-cohis
Plasmid#133433PurposeYeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationC59S/C252A, Codon optimized for P. pastoris, and …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mNeonGreen
Plasmid#124101PurposeExpression of the fluorescent protein mNeonGreen in mammalian cellsDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationCodon usage was optimized for Homo SapiensPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pTH849-gst-optec
Plasmid#89590PurposePlasmid for bacterial expression of His-tagged GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTH848-gst-disec
Plasmid#89589PurposePlasmid for bacterial expression of His-tagged GST encoded by non-preferred codonsDepositorArticleInsertglutathione-S transferase codon-dis-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9_VP64
Plasmid#49013Purposeencodes yeast-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (codon-optimized for expression in S. cerevisiae)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionYeastMutationPromoterpTPGI (galactose+aTc inducible)AvailabilityAcademic Institutions and Nonprofits only -
pCMV_dCas9_VP64
Plasmid#49015Purposeencodes human-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (human-codon-optimized)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pGC4780
Plasmid#113408PurposeTranslation reporter with inducible translation coupling (high native GFP expression)DepositorInsert158_33121123_1
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGC4785
Plasmid#113409PurposeTranslation reporter with inducible translation coupling (low native GFP expression, high under coupling)DepositorInsert359_31121113_2
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailabilityAcademic Institutions and Nonprofits only -
HeFm2SpCas9
Plasmid#92111PurposeExpression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)DepositorInsert“Highly enhanced Fidelity” mut2 SpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, K848A, Q926A, R1060APromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pX330-HA-dSpCas9
Plasmid#92112PurposeExpression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)DepositorInsertdead/inactive SpCas9
UseCRISPRTagsHA tag and NLSExpressionMammalianMutationD10A, H840APromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-dSpCas9
Plasmid#92113PurposeExpression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)DepositorInsertdead/inactive SpCas9 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, H840APromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-wtSpCas9 (without sgRNA)
Plasmid#92353PurposeExpression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFlag-NLS-wild-type SpCas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
UseTagsExpressionBacterialMutationPromotertrcAvailabilityAcademic Institutions and Nonprofits only
Showing: 1621 - 1640 of 7113 results