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Plasmid#124394PurposeE.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898E (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationT898EPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-T898A(pL91)
Plasmid#124393PurposeE.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationT898APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L897A(pL91)
Plasmid#124392PurposeE.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L897A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationL897APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-I894A(pL91)
Plasmid#124391PurposeE.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-I894A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationI894APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pIS58 TIP120A 3'UTR
Plasmid#14501DepositorInsertTIP120A 3'UTR (CAND1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
MCJ.559
Bacterial Strain#69493PurposeStrain with recoded TAG and genomic deletions for improved incorporationDepositorBacterial ResistanceAmpicillinSpeciesEschericia coliAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-NONO
Plasmid#127655PurposeOver-express HA-tagged human NONODepositorInsertNONO (NONO Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Nme2ABE8e
Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertNme2ABE8e
UseTagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagExpressionMutationPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-CjABE8e
Plasmid#189929PurposePlasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertCjABE8e
UseTagsExpressionMammalianMutationCjCas9 D8APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-P405A/P531A-pBabe-puro
Plasmid#19006DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: P405A and P531APromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1658-dCas9-EGFP
Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianMutationPromoterMSCV LTR promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-SauriABE8e
Plasmid#189927PurposePlasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertSauriABE8e
UseTagsExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha-wt-pBabe-puro
Plasmid#19365DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwt human cDNAPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_003
Plasmid#192505PurposeFor cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFPDepositorInserthU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-WT-STAT3
Plasmid#71450PurposeRetroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXG1
Plasmid#153124PurposeExpression of Flag tagged human FOXG1 in mammalian cellsDepositorInserthuman FOXG1 (FOXG1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBT-PE2-PuroTK-pegRNA_GG
Plasmid#173222PurposepiggyBac transposon containing both PE2 and a pegRNA cloning site.DepositorInsertsPE2
PuroTK
pegRNA cloning cassette
UsePiggybacTagsExpressionMammalianMutationPromoterCMV, P2A, and hU6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-human-BNIP3
Plasmid#100781PurposeMammalian expression of BNIP3DepositorInserthuman-BNIP3 (BNIP3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only