Addgene

We narrowed to 1,780 results for: ipp

Showing: 1601 - 1620 of 1780 results

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pLX307-VHL(C162F)-3F-BirA

Plasmid

#220149

Purpose

Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

changed cysteine 162 to phenylalanine; codon opti…

Promoter

EF-1a

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL(Y98H)-3F-BirA

Plasmid

#220148

Purpose

Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

changed tyrosine 98 to histidine; codon optimized

Promoter

EF-1a

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-Alox5-PLAT-EGFP-6H

Plasmid

#187189

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000057273 (alox5a Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-AKT1-PH-EGFP-6H

Plasmid

#187157

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000142208 (AKT1 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFL I3

Plasmid

#113514

Purpose

Expression of I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry_C1 I3(resist)

Plasmid

#61399

Purpose

Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations of bases C183T T192C T189C

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3(resist)

Plasmid

#61400

Purpose

Expresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

silent mutations of bases C183T T192C T189C

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3(V41A/W43A)

Plasmid

#61402

Purpose

Expresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

inhibitor-3 (PPP1R11 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

V41A/W43A

Promoter

CMV

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00123

Plasmid

#34277

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3851 (odd Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
BLADE-182

Plasmid

#134914

Purpose

sgRNA targeting GFP to be used in nanoblade system

Depositor

Philippe Mangeot, Emiliano Ricci, Théophile Ohlmann

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEB2-mScarlet-I

Plasmid

#104007

Purpose

Plasmid encoding mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet-I

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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iOn-CAG∞MCS

Plasmid

#154013

Purpose

Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

MCS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEB2-mScarlet

Plasmid

#104006

Purpose

Plasmid encoding mScarlet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-human APPL1

Plasmid

#22198

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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BLADE

Plasmid

#134912

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system

Depositor

Philippe Mangeot, Emiliano Ricci, Théophile Ohlmann

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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mRFP-human APPL1

Plasmid

#22202

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Use

Tags

mRFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pLX304-VHLdeltaB-IRES-GFP

Plasmid

#187642

Purpose

Mutant VHL Expression vector with GFP

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

VHL (VHL Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

deletion of amino acids 91 to 121

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEB2-mRuby3 Addgene

Plasmid

#104005

Purpose

Plasmid encoding mRuby3 Addgene

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRuby3 Addgene

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFL His-mEos3.2-I3

Plasmid

#113516

Purpose

Expression of His-Eos-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His-mEos3.2

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 CaRhAC T2A tDimer

Plasmid

#101722

Purpose

- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

Depositor

Thomas Oertner

Article

Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

Inserts

CatRhAC
red fluorescent protein

Use

AAV

Tags

Expression

Mutation

E497K,C569D

Promoter

human Synapsin1 promotor and ribosomal skip seque…

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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