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Plasmid#216019PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorInsertnCas9-SpG (H840A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA060
Plasmid#216017PurposeFragmid fragment: (Cas protein) nickase Cas for prime editingDepositorInsertnCas9 (H840A)_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA401
Plasmid#215959PurposeFragmid fragment: (guide cassette) guide expressionDepositorHas ServiceCloning Grade DNAInsertU6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA048
Plasmid#216016PurposeFragmid fragment: (Cas protein) deactivated CasDepositorInsertdCas12a (D908A)_v1.1 [EnAs]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA370
Plasmid#215988PurposeFragmid fragment: (N' terminus) attenuate expressionDepositorHas ServiceCloning Grade DNAInsertStart; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1D
Plasmid#196686PurposeRep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTFR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1B
Plasmid#196684PurposeRep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPSQGTLR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1A
Plasmid#196683PurposeRep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTVR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK265 (RAD21-mAC Hygro)
Plasmid#140539PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGG431
Plasmid#165615PurposeVector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
UseCRISPRTagsSV40 NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT3
Plasmid#164695PurposeExpression of PRMT3 with an N-terminal FLAG tagDepositorInsertPRMT3 (PRMT3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT3(E338Q)
Plasmid#164696PurposeExpression of PRMT3(E338Q) with an N-terminal FLAG tagDepositorInsertPRMT3 (PRMT3 Human)
UseTagsFLAGExpressionMammalianMutationE338QPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZE-PalkB-GFP
Plasmid#44381DepositorInsertsGFP
PalkB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPalkBAvailable sinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSF-KpAGO207-1251
Plasmid#117854PurposeExpressed KpAGO 207-1251 in E. coli under IPTG-inducible promotorDepositorInsertArgonaute
UseTags6xHis-SUMO encoded in vectorExpressionBacterialMutationmissing first 206 amino acid residuesPromoterT7Available sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGKO2
Plasmid#63617Purposea binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.DepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW071
Plasmid#140037PurposePCC 11901 fadD locus KO with a kanamycin resistance cassetteDepositorInsertKanamycin resistance gene
UseGene knockout in cyanobacteriaTagsExpressionMutationPromoterKanRAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG108 - K
Plasmid#178039PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markersDepositorInsertKanamycin resistance
UseTagsExpressionBacterialMutationErmAM has been replaced with Kanamycin for select…PromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDHt-SK
Plasmid#63615PurposeThe multiple cloning site of pGreenII0000 cloned into binary vector pDHtDepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDHt-KS
Plasmid#63616PurposeThe multiple cloning site of pGreenII0000 cloned into binary vector pDHtDepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only