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Plasmid#86947PurposeConstruct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.DepositorInsertAcGFP-progerin (LMNA Human)
UseAAVTagsAcGFPExpressionMammalianMutationprogerin sequence is LMNA gene with nucleotide C1…PromoterCMV and T7Available sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PACT
Plasmid#15667DepositorInsertPACT (PRKRA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTK88_GFP-Anillin
Plasmid#46354DepositorInsertAnillin (ANLN Human)
UseRetroviralTagsGFPExpressionMammalianMutationa1854 is changed to c to disrupt XhoI site withou…PromoterpBABEAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0271
Plasmid#142505PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF7 (TCF7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0211
Plasmid#142484PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXP3 (FOXP3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d_RspCasWYL1
Plasmid#108304PurposeExpresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.DepositorInsertsRspCas13d
RspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-A-NG
Plasmid#220895PurposeExpress haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-A-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nanog-2A-GFP
Plasmid#59994PurposeInserts GFP immediately downstream of the Nanog coding regionDepositorInsertNanog (Nanog Mouse)
UseMouse TargetingTags2A-GFPExpressionMutationPromoterAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC5A5_STOP
Plasmid#161348PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC5A5 (SLC5A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ132-tRNA
Plasmid#179211PurposeGolden Gate entry vector; 2nd gRNA with tRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterno promoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm4b-pA
Plasmid#104486PurposeExpress mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.DepositorInsertKdm4b (Kdm4b Mouse)
UseTagsFlag epitope tagExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3 Gag-imCherry
Plasmid#208882Purposecodes for Gag from HIV-1 with an internal mCherry fluorescent proteinDepositorArticleInsertHIV-1 Gag
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF HaloTag-SYB2
Plasmid#197282PurposeEncodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseTagsExpressionMammalianMutationQ76V and F77WPromoterEF1alphaAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B1-deltaE1-GFP
Plasmid#179203PurposeBlock 1 with deletion in E1 and insertion of GFP expression cassetteDepositorInsertModified Block 1 with deletion in E1 and insertion of GFP expression cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST
Plasmid#208041PurposeEnables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidinDepositorInsertTurboID
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2595
Plasmid#142337PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRORC (RORC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2970
Plasmid#144446PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRORC (RORC Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NM23-H1
Plasmid#157987PurposeExpresses NM23-H1 in E.coli cellDepositorInsertNM23-H1 (NME1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-Puro
Plasmid#115143PurposeSafe harbor site 231 knock-in vector with puromycin resistance selection cassetteDepositorInsertPuromycin
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i1
Plasmid#120882PurposeExpresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.DepositorInsertCas12i1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only