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Plasmid#117541PurposeLevel1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (LbCas12a)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0028
Plasmid#117540PurposeLevel1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (AsCas12a)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0022
Plasmid#117537PurposeLevel1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (Sp Cas9) - extended stem
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0012
Plasmid#117533PurposeLevel0 Golden Gate part: CDS, eSaCas9 (no stop codon)DepositorInserteSaCas9 (no stop codon)
UseCRISPRTagsExpressionMutationR499A,Q500K,R654A,G655RPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0018
Plasmid#117528PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.1DepositorInsertSp-eCas9 1.1 (with stop codon)
UseCRISPRTagsExpressionMutationK848A, K1003A, R1060APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0015
Plasmid#117525PurposeLevel0 Golden Gate part: CDS, SpCas9-KA (no stop codon)DepositorInsertSpCas9-KA (no stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0014
Plasmid#117524PurposeLevel0 Golden Gate part: CDS, SpCas9-KADepositorInsertSpCas9-KA (with stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0001
Plasmid#117523PurposeLevel0 Golden Gate part: CDS, SpCas9-DE (no stop codon)DepositorInsertSpCas9-DE (no stop codon)
UseCRISPRTagsExpressionMutationD1135EPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0009
Plasmid#117522PurposeLevel0 Golden Gate part: CDS, SpCas9-p (no stop codon)DepositorInsertSpCas9-p (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL90005
Plasmid#117520PurposeLevel0 Golden Gate part: CDS, SpCas9-h (no stop codon)DepositorInsertSpCas9-h (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0008
Plasmid#117535PurposeLevel0 Golden Gate part: CDS, AsCas12aDepositorInsertAsCas12a (with stop codon)
UseCRISPRTags3xHA and NLSExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF004
Plasmid#48845PurposeProvides a D-alanine resistance cassette as GreenGate module.DepositorInsertp35S::D-AlanineR::t35S
UseGolden gate compatible cloning vectorTagsExpressionMutationinternal BsaI recognition site in 35S promoter re…PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
GreenGate
Plasmid Kit#1000000036PurposeGolden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassetteDepositorAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
Platinum Gate TALEN Kit
Plasmid Kit#1000000043PurposeEnables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.DepositorAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B
Plasmid#69283PurposeGolden Gate entry vector; 3rd gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131B
Plasmid#69281PurposeGolden Gate entry vector; 1st gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B
Plasmid#69282PurposeGolden Gate entry vector; 2nd gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134B
Plasmid#179216PurposeGolden Gate entry vector; 4th gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAtU3Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-Cherry-sgRNA
Plasmid#221387Purposeepisomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAsDepositorInsertnone, cloning vector for introduction of targeting sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only