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Addgene
Showing: 1581 - 1600 of 7249 results

  1. pHH0103 STXBP4 WW domain

    Plasmid
    #104265
    Purpose
    Bacterial expression of WW domain from STXBP4
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    STXBP4 WW domain (STXBP4 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104265-plasmid-map-sequence-id-203219

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  2. pHH0103 PIN1L WW domain

    Plasmid
    #104269
    Purpose
    Bacterial expression of WW domain from PIN1L
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    PIN1L WW domain (PIN1P1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104269-plasmid-map-sequence-id-203206

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  3. pHH0103 UTROPHIN WW domain

    Plasmid
    #104275
    Purpose
    Bacterial expression of WW domain from UTROPHIN
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    UTROPHIN WW domain (UTRN Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104275-plasmid-map-sequence-id-203220

    Enlarge

  4. pAAV-CAG-mNeonGreen

    Plasmid
    #124101
    Purpose
    Expression of the fluorescent protein mNeonGreen in mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mNeonGreen
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Codon usage was optimized for Homo Sapiens
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    124101-plasmid-map-sequence-id-241203

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  5. pPICZaa-STE2-cohis

    Plasmid
    #133433
    Purpose
    Yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S/C252A, Codon optimized for P. pastoris, and …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133433-plasmid-map-sequence-id-267727

    Enlarge

  9. pTE3330

    Plasmid
    #107534
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R
    Promoter
    CMV and human U6
    Availability
    Academic Institutions and Nonprofits only
    107534-plasmid-map-sequence-id-207314

    Enlarge

  10. pTE3327

    Plasmid
    #107535
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RVR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K548V, N552R
    Promoter
    CMV and human U6
    Availability
    Academic Institutions and Nonprofits only
    107535-plasmid-map-sequence-id-207331

    Enlarge

  11. pTE3331

    Plasmid
    #107536
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K613V, N617R
    Promoter
    CMV and human U6
    Availability
    Academic Institutions and Nonprofits only
    107536-plasmid-map-sequence-id-207338

    Enlarge

  12. pTE3334

    Plasmid
    #107537
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K582V, N586R
    Promoter
    CMV and human U6
    Availability
    Academic Institutions and Nonprofits only
    107537-plasmid-map-sequence-id-207346

    Enlarge

  13. pTE3329

    Plasmid
    #107538
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    G532R, K595R
    Promoter
    CMV and human U6
    Availability
    Academic Institutions and Nonprofits only
    107538-plasmid-map-sequence-id-207235

    Enlarge

  14. pTE3328

    Plasmid
    #107539
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K607R
    Promoter
    CMV and human U6
    Availability
    Academic Institutions and Nonprofits only
    107539-plasmid-map-sequence-id-207236

    Enlarge

  17. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

    Plasmid
    #157701
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    157701-plasmid-map-sequence-id-307865

    Enlarge

  18. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid
    #157702
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    157702-plasmid-map-sequence-id-307866

    Enlarge

Showing: 1581 - 1600 of 7249 results