168,579 results

1. pBAD/HisB (TIREVOL)-mEos3.2

   Plasmid

   #189720

   Purpose

   titratable expression of mEos3.2 in bacteria

   Depositor

   Daniel Daley

   Article

   Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

   Insert

   mEos3.2
   

   Tags

   His-T7-EK

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-HCMVgH-8his (TB40/E)

   Plasmid

   #136445

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Tags

   Short GGSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular domain (a.a. 1-717). Codon-optimize…

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-HCMVgO-8his (TB40/E)

   Plasmid

   #136453

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   envelope glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Tags

   Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF1863

   Plasmid

   #143327

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU2F2 (POU2F2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GB4068

   Plasmid

   #187810

   Purpose

   Module for the constitutive expression of Cup2:Gal4AD and copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).

   Depositor

   Diego Orzaez

   Article

   Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

   Insert

   copper-inducible dCasEV2.1
   

   Expression

   Plant

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FOXM1 C11.2 gRNA

   Plasmid

   #90690

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FOXM1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   FOXM1 (Guide Designation C11.2)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCVA-amiR-PDS

   Plasmid

   #32911

   Depositor

   Yule Liu

   Article

   Tang et al Plant Physiol. 2010 Jun;153(2):632-41. E

   Insert

   amiR-PDS (Nb)
   

   Use

   Plant expression

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. psiCHECK2-AS34a

   Plasmid

   #37099

   Depositor

   Judy Lieberman

   Article

   Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

   Insert

   perfect complementary binding site for hsa-miR-34a (MIR34A Human)
   

   Use

   Luciferase

   Available Since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-VTKS4-dTom

   Plasmid

   #178718

   Purpose

   AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   dTom
   

   Use

   AAV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AeU6 3p3 GFP LgRNA

    Plasmid

    #182208

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3xP3-GFP
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF3434

    Plasmid

    #144910

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF37A (ZNF37A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TP53-KO_gRNA_2

    Plasmid

    #195131

    Purpose

    TP53-targeting gRNA in the LRG2.1 backbone

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    TP53 KO gRNA 2 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TNPO1-shRNA

    Plasmid

    #86082

    Purpose

    A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET15b-pknG

    Plasmid

    #102814

    Purpose

    Expression of PknG in E.coli

    Depositor

    Jean Pieters

    Article

    Walburger et al Science. 2004 Jun 18;304(5678):1800-4. E

    Insert

    pknG
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF0822

    Plasmid

    #142230

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LHX6 (LHX6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF2078

    Plasmid

    #141910

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT2Apuro-TRE-synPCB2.0

    Plasmid

    #139481

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Use

    Tol2 transposon donor vector

    Expression

    Mammalian

    Promoter

    Tet responsive element

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.TRC1.shmNsd1.1, puro

    Plasmid

    #114446

    Purpose

    Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

    Depositor

    Adrian Bracken

    Article

    Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

    Insert

    shNsd1.1 (Nsd1 Mouse)
    

    Use

    Lentiviral and RNAi

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHES553 [prGal1-RAS2(KAN) pnh604]

    Plasmid

    #103846

    Purpose

    Yeast Expression Plasmid

    Depositor

    Hana El-Samad

    Article

    Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

    Insert

    pGal1-Ras2(KAN)
    

    Expression

    Yeast

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175784

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge