168,838 results

1. HeLa full-length MLCK-GFP

   Plasmid

   #46316

   Depositor

   Anne Bresnick

   Article

   Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

   Insert

   HeLa long myosin light chain kinase (MYLK Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-FLEX-EGFP

   Plasmid

   #59331

   Purpose

   Expresses EGFP

   Depositor

   Ian Wickersham

   Has Service

   AAV1

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pv6_CBX1_2xChromo

   Plasmid

   #179393

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

   Plasmid

   #224501

   Purpose

   Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   Lamin B Vhh mNeonGreen-pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAH01Sp::Cas9

   Plasmid

   #128160

   Purpose

   Ptet-controlled expression of S. pyogenes Cas9

   Depositor

   Calvin Henard

   Article

   Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

   Insert

   Sp Cas9
   

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP11457 - pAAV-eHGT_078h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1457)

   Plasmid

   #163495

   Purpose

   Direct-expressing SYFP2 AAV Virus

   Depositor

   Allen Institute for Brain Science, Boaz Levi

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Promoter

   minBetaGlobin

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2582

   Plasmid

   #142436

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR1D1 (NR1D1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SUMO-PNGaseF

   Plasmid

   #228498

   Purpose

   10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   ngl
   

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xFLAG-CUL5-pCMV7.1

   Plasmid

   #155024

   Purpose

   N-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 5 (CUL5 Human)
   

   Tags

   3xFLAG

   Expression

   Bacterial

   Promoter

   pCMV7.1

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV x EF1a_EKAREN5(TA)-ires-puro

    Plasmid

    #167823

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP-DAT

    Plasmid

    #90228

    Purpose

    Expresses human DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221_SLC19A1

    Plasmid

    #132266

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC19A1 (SLC19A1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCVL SFFV-EF1s HA.NLS.Sce(opt)

    Plasmid

    #31479

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV/Ef1s HA NLS I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    "opt" indicates the coding sequence has…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRESpuroN3xFLAG-UFSP2 C302A

    Plasmid

    #204554

    Purpose

    Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFSP2 (UFSP2 Human)
    

    Expression

    Mammalian

    Mutation

    C302A

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

    Plasmid

    #231372

    Purpose

    Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

    Depositor

    David Bartel

    Article

    Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

    Insert

    Human Argonaute2 (AGO2 Human, Synthetic)
    

    Tags

    3xFLAG-SUMO_Eu1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET Trx His6 LIC cloning vector (2Tc-T)

    Plasmid

    #37239

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis and Thioredoxin (Trx)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFl Strep-sumo-TEV-hAgo2

    Plasmid

    #136235

    Purpose

    Expresses human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Tags

    Strep Sumo

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p667-UBC-CDK12-V5_IDG-K

    Plasmid

    #135245

    Purpose

    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK12-V5 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23119

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid

    #115161

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

    Inserts

    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    

    Use

    AAV

    Tags

    hM3Dq-mCherry

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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