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Plasmid#133446PurposeEscherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selectionDepositorTypeEmpty backboneUseE.coli/s.aureus shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only
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pdCas9-J23109
Plasmid#113151PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A & H840APromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV5)
Viral Prep#162381-AAV5PurposeReady-to-use AAV5 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV8)
Viral Prep#28306-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-mNeonGreen
Plasmid#137804PurposeVisualization of the Endoplasmic ReticulumDepositorArticleInsertKDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag
Plasmid#208051PurposeEmpty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with FlagDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-A3-HA-ubiquitin
Plasmid#220145PurposeExpresses A3-Ub for E3 substrate identification by E-STUBDepositorInsertUbiquitin C (UBC Human)
UseTagsA3-HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330A-nBRD4/PITCh
Plasmid#91794PurposePITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4DepositorInsertSpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterchicken β-actin promoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sfp pet29b C-terminal His Tag
Plasmid#75015PurposeExpresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACPDepositorInsertSfp
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE50
Plasmid#90389PurposePPARg/RARa - PPARg gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterDR1Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR OVA/p2a/mCherry-CaaX
Plasmid#113030Purposefor lentivirus production of ovalbumin and mcherry-caaxDepositorInsertOVA/p2a/mCherry-CaaX
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_3xFlagEzh2 WT
Plasmid#173717PurposeWT EZH2 expression plasmid with 3XFLAG tagDepositorInsertEZH2 (EZH2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-MagLOV
Plasmid#219957PurposeBacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2DepositorInsertMagLOV
UseTags6xHisExpressionBacterialMutationAsLOV2 C450P L496V Q513K G528K D540MPromoterT7Available sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27
Plasmid#22902DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK27 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27
Plasmid#22902DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK27 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMH-Halo tag
Plasmid#154144PurposeGateway compatible mammalian expression plasmid for halo tag fusion proteinsDepositorTypeEmpty backboneUseTagsHalo tagExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only