165,173 results

1. pTalpha1-Dre

   Plasmid

   #133925

   Purpose

   Neuron-specific expression of Dre

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   HA-Dre
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK-PUM1-rABE-Flag

   Plasmid

   #191385

   Purpose

   Doxycycline inducible expression of PUM1-rABE

   Depositor

   Stephen Floor

   Article

   Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

   Insert

   PUM1-rABE (PUM1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   tet-inducible promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-6011nes

   Plasmid

   #108652

   Purpose

   An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-ERK-EV
   

   Use

   Tags

   XFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGCS vector kit

   Plasmid Kit

   #1000000107

   Purpose

   A series of pCS2+ based Gateway vectors with N- or C-terminal tags (eg Myc, HA, Flag, GST and eGFP), which allow the generation of tagged proteins in a variety of vertebrate organisms.

   Depositor

   Hai-Ning Du

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

5. TGFbetaR1 (T204D)-pcw107-V5

   Plasmid

   #64629

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   TGFBR1 (transcript variant 1) (TGFBR1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   T204D

   Promoter

   PGK

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLENTI EF1alpha FLAG/HA-PALI1

   Plasmid

   #114445

   Purpose

   Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   PALI1 (LCOR Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDC216

   Plasmid

   #115791

   Purpose

   Expresses rat Syntaxin 1

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   Syntaxin1 (Stx1a Rat)
   

   Use

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   C145S S193C C271S C272S

   Promoter

   T7

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. epi-BE4max-NG

   Plasmid

   #135977

   Purpose

   Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

   Depositor

   Yongming Wang

   Article

   Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

9. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

   Plasmid

   #224536

   Purpose

   A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG- NLS-mScarlet-I3
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSWAP_Entry_H2_beta_CM

    Plasmid

    #184870

    Purpose

    pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHiUGE GS-gRNA (hSyn-Cre) backbone

    Plasmid

    #200368

    Purpose

    Backbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p3xFLAG-CMV10-hUVRAG

    Plasmid

    #24292

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV irradiation resistance-associated gene (UVRAG Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

13. p23-puro-EGFP-RPA194

    Plasmid

    #179237

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    RPA194 (POLR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-51b(+)_NAD-Snifit

    Plasmid

    #113658

    Purpose

    Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NAD-Snifit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWZL Neo Myr Flag DLG5

    Plasmid

    #20477

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    DLG5 (DLG5 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pHiUGE smFP-V5 donor ORF0

    Plasmid

    #200380

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHiUGE smFP-V5 donor ORF1

    Plasmid

    #200381

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTol1:uas-myc-act-map2k2b-2a-tdTomato

    Plasmid

    #104721

    Purpose

    UAS-driven expression of activated MEK

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated map2k2b (map2k2b Zebrafish)
    

    Use

    Zebrafish transposon

    Tags

    2A-tdTomato and myc tag

    Expression

    Mutation

    serines 221 and 225 to aspartic acid

    Promoter

    10xUAS

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1 mTagBFP2 rat PGK1 shRNA

    Plasmid

    #222869

    Purpose

    Expresses mTagBFP2 along with an shRNA against rat PGK1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1 shRNA (Pgk1, Pgk1 Mouse, Rat)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSWAP_Entry_H1_alpha

    Plasmid

    #184865

    Purpose

    pSwap plasmid part with H1_alpha homology arms

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge