169,008 results

1. pJOP-HTT-HR81Q

   Plasmid

   #92250

   Purpose

   HTT donor DNA with 81Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMW41: pET-His-MBP-SUMO-R2Bm-SII

   Plasmid

   #209290

   Purpose

   Expresses the R2Bm ORF in E. coli

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2
   

   Tags

   His, MBP, StrepII, and bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. cBEST4

   Plasmid

   #234660

   Purpose

   Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

   Depositor

   Mingzi M. Zhang

   Article

   Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

   Inserts

   spCas9 cytosine base editor
   Golden Gate compatible sgRNA insertion cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3 and tcp830

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-FRT/T0-GFP-PALB2

   Plasmid

   #71113

   Purpose

   mammalian expession of GFP-PALB2

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   PALB2 (PALB2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV/TO

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAG426GAL-EGFP-ccdB

   Plasmid

   #14323

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_pLyn_EGFP

   Plasmid

   #214605

   Purpose

   Expresses fluorescent protein EGFP targeted to plasma membrane

   Depositor

   Peter Dedecker

   Article

   Valenta et al Talanta. 2024 Mar

   Insert

   lyn-EGFP
   

   Tags

   lyn targetting

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

   Plasmid

   #197031

   Purpose

   Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized denOsCas12f1-VPR
   

   Expression

   Mammalian

   Mutation

   D52R / T132R / D228A / D406A

   Promoter

   CBh, CMV, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0037

   Plasmid

   #141488

   Purpose

   Lentiviral vector for overexpressing the SP5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SP5 (SP5 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-Dual-iCas9-EFS

   Plasmid

   #167501

   Purpose

   Expression of dual inducible Cas9 with EFS promoter

   Depositor

   Jake Liang

   Article

   Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   Cas9
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]

    Plasmid

    #218344

    Purpose

    Donor plasmid of MAG Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC-6-BeYDV-Rep-RepA-9

    Plasmid

    #197715

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV Rep/RepA proteins: native ORFs
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GPR116-Tango

    Plasmid

    #66307

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR116 (ADGRF5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pBT13-splitD

    Plasmid

    #122597

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMEV4

    Plasmid

    #110539

    Purpose

    The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

    Depositor

    William B. Whitman

    Article

    Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pFlare9A-Dup34

    Plasmid

    #90267

    Purpose

    Minigene reporter for alternative splicing analysis

    Depositor

    Douglas Black

    Article

    Stoilov et al Proc Natl Acad Sci U S A. 2008 Aug 12;10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

    Plasmid

    #51094

    Purpose

    Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    oChIEF(E163A/T199C)
    

    Use

    AAV

    Tags

    P2A-dTomato

    Expression

    Mammalian

    Mutation

    E163A/T199C

    Promoter

    EF1a

    Available Since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLKO.1-G3BP1-3'UTR

    Plasmid

    #136038

    Purpose

    G3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSynapsin- soCoChR-GFP

    Plasmid

    #107708

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Has Service

    AAV9

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgLATS1/2-2

    Plasmid

    #229432

    Purpose

    knockout of LATS1 and LATS2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgLATS1/2-2 (LATS1, LATS2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6;bU6;EFS

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG424GAL-ccdB

    Plasmid

    #14151

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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