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Plasmid#25147PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertusp21.209.563 (USP21 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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UBH26
Plasmid#25467PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertubh26.019.099 (UBQLN3 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 19-99PromoterAvailable sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAAV-i53
Plasmid#92170Purposerecombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1DepositorInserti53
UseAAVTagsFLAGExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJuly 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-N-Myc_DED/AAA 535-888 USP7
Plasmid#131255PurposeMammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-888 USP7 ā¦PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX307-CRBN-3F-BirA
Plasmid#220146PurposeExpresses CRBN-BirA for CRBN substrate identification by E-STUBDepositorInsertCereblon (CRBN Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(C162F)-3F-BirA
Plasmid#220149PurposeExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged cysteine 162 to phenylalanine; codon optiā¦PromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(Y98H)-3F-BirA
Plasmid#220148PurposeExpresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged tyrosine 98 to histidine; codon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-ISG15 C78SN13Y
Plasmid#165106Purposebacterial expressio of a GST fusion of Human ISG15 C78SN13YDepositorInsertISG15 (ISG15 Human)
UseTagsGST from plasmid.ExpressionBacterialMutationC78SN13Y; The sequence of ISG15-C78S/N13Y contaiā¦PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1_6xHis-TEV-eGFP-P62
Plasmid#190929PurposePlasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390DepositorInsertP62 (SQSTM1 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-OPTN WT
Plasmid#190191PurposePlasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797DepositorInsertoptineurin (OPTN Human)
UseTags6xHis, TEV, and mCherryExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfast bac-flag Fbxl3
Plasmid#51894PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorInsertFbxl3 (Fbxl21 Mouse)
UseTagsFLAGExpressionMutationPromoterpolyhedrin promoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-6xHis-TEV-NDP52
Plasmid#190194PurposePlasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401DepositorInsertNDP52 (CALCOCO2 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOE-E3-T2A-mCherry
Plasmid#190808PurposeExpress APOE E3 allele from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE3PromoterUbi CAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOE-E4-T2A-mCherry
Plasmid#190809PurposeExpress APOE E4 allele from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE4PromoterUbi CAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOE-E2-T2A-mCherry
Plasmid#190807PurposeExpress APOE E2 allele from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE2PromoterUbi CAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-2xV5-TurboID-NES-STOP_IDG-K
Plasmid#158162PurposeGateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsert2xV5-TurboID-NES
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only