Addgene

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mOrange pHis-//

Plasmid

#53304

Purpose

Empty vector to express N-terminal His-mOrange-tagged fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Type

Empty backbone

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LRG2.1-mOrange

Plasmid

#124772

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pActinin-LSSmOrange

Plasmid

#37139

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human alpha-actinin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD-PSmOrange2

Plasmid

#34957

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Use

Tags

Expression

Bacterial

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Promoter

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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OCT4-2A-mOrange

Plasmid

#66986

Purpose

Generate OCT4 mOrange reporter line without selection cassette

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

2A-mOrange

Use

Tags

Expression

Mutation

Promoter

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A (S-A)

Plasmid

#8825

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A inactive (GZMA Human)

Use

Tags

(His)6

Expression

Bacterial

Mutation

changed Serine 184 to Alanine

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
pJL1-mOrange

Plasmid

#102632

Purpose

In vitro expression of mOrange from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mOrange

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pH2B-LSSmOrange

Plasmid

#37133

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

histone 2B

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/pr-Membrane

Plasmid

#120817

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

pr-Membrane ZIKV

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX3X-RanBP2dFG

Plasmid

#53120

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

RanBP2 fragment (RANBP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2553-2838)

Promoter

tac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pE2-Orange-N1

Plasmid

#21719

Depositor

Benjamin Glick

Article

Strack et al BMC Biotechnol. 2009 . 9():32.

Insert

E2-Orange

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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H2B-Orange

Plasmid

#20969

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Use

Tags

Orange

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pVimentin-PSmOrange

Plasmid

#31922

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Vimentin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pUCP20T-morange

Plasmid

#78468

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

mOrange

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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hCCR5-LSSmOrange

Plasmid

#110194

Purpose

Encodes for human CCR5 coding sequence tagged with the LSS-mOrange FP

Depositor

Sergi Padilla Parra

Article

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Insert

hCCR5 (CCR5 Human)

Use

Tags

LSSmOrange

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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BFP-RanQ69L

Plasmid

#163438

Purpose

Mammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredation

Depositor

Sanford Simon

Insert

RanQ69L (RAN Human)

Use

Tags

tag-BFP

Expression

Mammalian

Mutation

Q69L

Promoter

CMV

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+FucciOrange

Plasmid

#128427

Purpose

Contains insert allowing in-vivo visualization of Cdk1 protein (G1)

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

FucciOrange

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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TfR-pHoran4

Plasmid

#61547

Purpose

Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

Depositor

David Perrais

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

Transferrin receptor (TFRC Human)

Use

Tags

pHoran4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pDisplay-pHoran4

Plasmid

#61557

Purpose

Orange fluorescent protein based pH indicator in pDisplay vector

Depositor

Robert Campbell

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHoran4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only