Addgene

We narrowed to 4,474 results for: puc

Showing: 141 - 160 of 4474 results

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pUC19-U6-crRNA4n96

Plasmid

#123640

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

crRNA scaffold 4n96

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pUCdeltaSS-ERE

Plasmid

#108217

Purpose

Yeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expression

Depositor

Keith Yamamoto, Didier Picard

Article

Picard et al Nature 1990 Nov 8;348(6297):166-8.

Insert

ERE-CYC1-lacZ

Use

Tags

Expression

Yeast

Mutation

Promoter

CYC1

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19_Ntag_BbsI-Mammalian

Plasmid

#186674

Purpose

N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Mammalian N-terminal Tag

Use

Tags

3xFlag-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-Q927P4

Plasmid

#103131

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))

Use

CRISPR; Cloning vector

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_FR13_APikL2

Plasmid

#123648

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

FR13_APikL2

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19/N-eCFP

Plasmid

#183155

Purpose

Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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SCP1-pUC119

Plasmid

#21846

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP1 (super core promoter 1)-pUC119

Use

Puc derivative - a cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Use

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-8xHis

Plasmid

#101699

Purpose

8xHis in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

AATG-[8xHis]-AGCC

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-THG73tRNA

Plasmid

#21292

Depositor

Henry Lester

Article

Saks et al J Biol Chem. 1996 Sep 20. 271(38):23169-

Insert

T. thermophila tRNA Glutamine (CUA)-G73

Use

T. thermophila

Tags

Expression

Mutation

N/A

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_GY11_00131271

Plasmid

#121355

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

GY11_00131271

Use

Golden gate

Tags

Expression

Mutation

Magnaporthe oryzae

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19/DdmRuby3

Plasmid

#154305

Purpose

cloned with the codon-optimized mRuby3 gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mRuby3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AagRNA

Plasmid

#121953

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b tracrRNA/crRNA duplex

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_FR13_00004061

Plasmid

#121292

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

FR13_00004061

Use

Golden gate

Tags

Expression

Mutation

Magnaporthe oryzae

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19/CF-mChe

Plasmid

#183166

Purpose

Construct for transient expression of cytoplasmic foci-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

CF-mCherry

Use

Tags

AtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1

Expression

Plant

Mutation

Promoter

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/Tp-mChe

Plasmid

#183165

Purpose

Construct for transient expression of tonoplast-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

Tp-mCherry

Use

Tags

28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/Pt-mChe

Plasmid

#183164

Purpose

Construct for transient expression of plastids-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

Pt-mCherry

Use

Tags

57 aa of the Pisum sativum rubisco small subunit …

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-C

Plasmid

#183160

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-K1LM04

Plasmid

#103119

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)

Use

CRISPR; Cloning vector

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-HHtRNA

Plasmid

#37222

Depositor

Jon Lorsch

Article

Acker et al Methods Enzymol. 2007;430:111-45.

Insert

HHtRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2012

Availability

Academic Institutions and Nonprofits only