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Showing: 141 - 160 of 5211 results

  3. pCAGmRuby2smFPMyc

    Plasmid
    #71816
    Purpose
    encodes multiple copies of an epitope tag Myc ( not detected by anti GFP ab)
    Depositor
    Loren Looger
    Insert
    mRuby2_smFP_Myc
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    71816-plasmid-map-sequence-id-422684

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  5. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  10. pCAGmRuby2smFPV5

    Plasmid
    #71817
    Purpose
    encodes multiple copies of an epitope tag V5( not detected by anti GFP ab)
    Depositor
    Loren Looger
    Insert
    mRuby2_smFP_V5
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  14. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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  15. pCas1_Cas2/3

    Plasmid
    #89240
    Purpose
    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1
    Depositor
    Blake Wiedenheft
    Article
    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p
    Insert
    Cas1 and Cas2/3
    Use
    Tags
    Strep II tag on Cas1
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89240-plasmid-map-sequence-id-176381

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Showing: 141 - 160 of 5211 results