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Plasmid#162942PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteinsDepositorInsertmonomeric Teal fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE
Plasmid#159945Purposeoptical regulation of nuclear exportDepositorInsertNLS-mTFP1.0-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)
Plasmid#101299PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 and 698-906 of alpha-C…PromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)
Plasmid#101297PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-906 of alpha-CateninPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)
Plasmid#101298PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 of alpha-CateninPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1
Plasmid#182380PurposeDual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promotersDepositorInsertsUseTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterCMV promoter and SV40 promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP12965 - pAAV-AiE0444h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2965)
Plasmid#191729PurposeAI plasmid ALIAS: AiP12965. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatumInsertmTFP1
UseAAVTagsExpressionMutationnonePromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP12700 - pAAV-AiE0452h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2700)
Plasmid#191708PurposeAI plasmid ALIAS: AiP12700. Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)InsertmTFP1
UseAAVTagsExpressionMutationnonePromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP13808 - pAAV-AiE0140h_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3808)
Plasmid#224176PurposeAiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neurons (note cortical Pvalb+ and L5_ET)InsertmTFP1
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP13754 - pAAV-AiE0779m_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3754)
Plasmid#191733PurposeAI plasmid ALIAS: AiP13754. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)InsertmTFP1
UseAAVTagsExpressionMutationnonePromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-CMVp-mito-mTFP1
Plasmid#182378PurposeDual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promotersDepositorInsertsUseTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterCMV promoter and SV40 promoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP12547 - pAAV-hsA2-AiE0140h-minRho-mTFP1-WPRE3-BGHpA (Alias: CN2547)
Plasmid#224061PurposeAiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neuronsInsertmTFP1
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
NK676
Plasmid#120852PurposeReporter gene expression in S. rosettaDepositorInsertpac-P2A-mTFP
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
NK696
Plasmid#120855PurposeReporter gene expression in S. rosettaDepositorInsertpac-P2A-mTFP-Jumble
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
NK694
Plasmid#120853PurposeReporter gene expression in S. rosettaDepositorInsertpac-P2A-Jumble-mTFP
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
NK697
Plasmid#120856PurposeReporter gene expression in S. rosettaDepositorInsertpac-P2A-mTFP-Jumblelw1
UseChoanoflagellate expressionTagsExpressionMutationJumblelw1 allele: T to C at nt 1109 causes L305P …PromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
NK695
Plasmid#120854PurposeReporter gene expression in S. rosettaDepositorInsertpac-P2A-Jumblelw1-mTFP
UseChoanoflagellate expressionTagsExpressionMutationJumblelw1 allele: T to C at nt 1109 causes L305P …PromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbChT (AAV9)
Viral Prep#45186-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry, mTFP, or noneAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK804
Plasmid#166058PurposePuromycin resistance and MRLC-mTFP expression for Salpingoeca rosettaDepositorInsertMRLC-mTFP
UseS. rosetta expressionTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
BSP609
Plasmid#122254Purposeexpression vector for mTFP under EFT3 promoterDepositorInsertmTFP
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only