-
Plasmid#81100PurposeGene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTT-y
Plasmid#81110PurposeGene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTU-y
Plasmid#81095PurposeGene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAM66
Plasmid#86478PurposeExpresses p9-mCitrineDepositorInsertp9-mCitrine
UseSynthetic BiologyTagsmCitrineExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAM12
Plasmid#86471PurposeExpresses p12 and p9-mCitrineDepositorInsertp12, p9-mCitrine
UseSynthetic BiologyTagsmCitrineExpressionBacterialMutationPromoterpBADAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAM53
Plasmid#86477PurposeExpresses p12 and mCitrine-p9DepositorInsertp12, mCitrine-p9
UseSynthetic BiologyTagsmCitrineExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
JD180
Plasmid#229486PurposeAdenoviral helper plasmid containing inducible E2A and L4-22k/33k genesDepositorInsertE2A, L4-22k/33k, mCitrine
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterTre (E2A, L4-22k/33k), ETR (mCitrine)Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM29
Plasmid#135489PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCitrineDepositorInserthph SpunH2A/Bpr hph-mCitrine
UseTagsmCitrineExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC230
Plasmid#118871Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mCitrine, cl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC231
Plasmid#118885Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease and mCitrine…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T2A-H2B mCitirine
Plasmid#210471PurposeCoexpression of Cas9 and H2B mCitrineDepositorInsertCas9-T2A-H2B mCitirine
UseCRISPR and Synthetic BiologyTagsmCitrine on C terminal of H2BExpressionMammalianMutationCas9 contains 3xFLAG tag and nuclear localization…PromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF138
Plasmid#135923PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mCitrine
UseTransposonTagsmCitrineExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mCit-IRES-Neo
Plasmid#155230Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mCitrine sensor
UseLentiviralTagsmCitrineExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gi-c010
Plasmid#173080PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGAP
UseTagsmCitrineExpressionBacterialMutationmCitrine + last 10 residues of GNA1, codon optimi…PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GgVcl 1-1066 FRET tail probe 2
Plasmid#46282DepositorInsertVcl 1-1066 + mCitrine (VCL Chicken)
UseTagsECFPExpressionMammalianMutationmCitrine (and linker) inserted after aa883PromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBGW
Plasmid#62665PurposeDonor vector for mCitrine knock-in via landing padDepositorInsertmCitrine
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
mCit(S1-S3)3-SAH
Plasmid#228067PurposeMammalian expression of coiled coil segments (S1-S3)x3, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCitrine, S1, S3
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only