Addgene

We narrowed to 573 results for: loxP

Showing: 141 - 160 of 573 results

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LoxP2-H2B-EGFP-V5-2A (SO107)

Plasmid

#99617

Purpose

To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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LoxP3-HA-H2B-Cerulean-2A (SO104)

Plasmid

#99618

Purpose

To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSL2-DHFR-mCherry-loxP.dna

Plasmid

#166235

Purpose

Floxed DHFR fused with mCherry

Depositor

David Sibley

Insert

mCherry

Use

Tags

Expression

Mutation

none

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P1-LoxP-Neo-N-miRFP670-C

Plasmid

#159434

Purpose

CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

Depositor

Jianhua Xing

Article

Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

Inserts

Neomycin
miRFP670

Use

CRISPR

Tags

3' linker pair 1, 5' linker pair 1, C t…

Expression

Mutation

Promoter

SV40

Availability

Academic Institutions and Nonprofits only

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YW249_Pzipt17-loxP-H2BGFP-miniMOS

Plasmid

#196063

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

H2B::GFP
NeoR
Minimal Mos1
zipt17p::zipt17::zipt17 3UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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YW347_Ptbb2UTR-loxP-RFP-miniMOS

Plasmid

#196064

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

myo2p driven tagRFP
NeoR
Minimal Mos1

Use

Tags

Expression

Worm

Mutation

Promoter

myo-2

Availability

Academic Institutions and Nonprofits only

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pTKEI-Dest-loxP-sfGFP

Plasmid

#202715

Purpose

Empty expression vector containing Cre recombinase reporter.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Pw7-loxP-term-loxP-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-loxP-canisNrg1

Plasmid

#173849

Purpose

Encoding dog Nrg1 before Cre-mediated recombination.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Nrg1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLenti-ePEG-LINE1-loxPsym

Plasmid

#216866

Purpose

Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

Depositor

Leopold Parts

Insert

spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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p1071-Lenti-Single loxP

Plasmid

#217892

Purpose

Lentiviral vector, entry vector for Lentiviral Switch-OVER

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

Plasmid

#62954

Purpose

Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

Plasmid

#62955

Purpose

Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

Plasmid

#62956

Purpose

Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Expression

Mutation

Promoter

none

Availability

Academic Institutions and Nonprofits only

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pBW707_pCAG-PV3-FRT-loxP-mCherry

Plasmid

#87571

Purpose

PV3 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

Plasmid

#87577

Purpose

PV4 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBW807_pCAG-PV4-loxP-mCherry-BGHpA

Plasmid

#87583

Purpose

PV4 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-3xHA-HXGPRT-LoxP

Plasmid

#86553

Purpose

used for a TIR1-AID system with CRISPR tagging technology in Toxoplasma gondii

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-3xHA-HXGPRT 3'UTR

Use

Expression in toxoplasma gondii

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLinker-BirA-3xHA-HXGPRT-loxP

Plasmid

#86668

Purpose

for generation of BirA tagged strains using CRISPR tagging technology

Depositor

David Sibley

Insert

Linker-BirA-3xHA-HXGPRT 3'UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-3xHA-DHFR-LoxP

Plasmid

#86669

Purpose

for generation of AID strains using CRISPR tagging technology

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-3xHA-HXGPRT 3'UTR

Use

Expression in toxoplasma gondii

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge