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Showing: 141 - 160 of 219 results

  1. AAVS1-eTLR

    Plasmid
    #214737
    Purpose
    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    AAVS1-eTLR (PPP1R12C Synthetic, Bovine, CMV, Anguilla japonica, influenza, Rabbit, Chicken, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214737-plasmid-map-sequence-id-425005

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  2. Negative Control Binder

    Plasmid
    #179140
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Use
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9. Addi…
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179140-plasmid-map-sequence-id-353410

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  5. pCEP4-HA-Flag-natT1R2 FS10

    Plasmid
    #113956
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, S212N substitution…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113956-plasmid-map-sequence-id-215284

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  6. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid
    #113953
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S, S212N, and …
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113953-plasmid-map-sequence-id-215167

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  7. pCEP4-HA-Flag-natT1R2 FS1

    Plasmid
    #113955
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, one base pair dele…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113955-plasmid-map-sequence-id-215169

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  8. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

    Plasmid
    #113952
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S and S212N su…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113952-plasmid-map-sequence-id-215166

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  9. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid
    #113957
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    residues 22-568 fused to a transmembrane tether
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113957-plasmid-map-sequence-id-215285

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  11. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Depositor
    Jacob Yount, Howard Hang
    Article
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Insert
    IFITM3 (Ifitm3 Mouse)
    Use
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST

    Plasmid
    #182547
    Purpose
    Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificity
    Depositor
    Robert de Vries
    Article
    Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e
    Insert
    H5_Vietnam_1203_04_Y161A
    Use
    Tags
    GCN4-TEV-sfGFP-TwinStrep
    Expression
    Mammalian
    Mutation
    Y161A mutant that confers exclusive NeuAc to NeuG…
    Promoter
    CMV
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182547-plasmid-map-sequence-id-362383

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  17. pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

    Plasmid
    #181980
    Purpose
    Human RUNX1C gene overexpression
    Depositor
    Jan-Henning Klusmann
    Insert
    RUNX1C (human) (RUNX1 Human)
    Use
    Lentiviral
    Tags
    HA (Human influenza hemagglutinin)
    Expression
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    181980-plasmid-map-sequence-id-358851

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  19. pRRL.PPT.SFFV.HA.RUNX1A.IRES.eGFP

    Plasmid
    #181979
    Purpose
    Human RUNX1A gene overexpression
    Depositor
    Jan-Henning Klusmann
    Insert
    RUNX1A (human) (RUNX1 Human)
    Use
    Lentiviral
    Tags
    HA (Human influenza hemagglutinin)
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    181979-plasmid-map-sequence-id-358973

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Showing: 141 - 160 of 219 results