Addgene

We narrowed to 51,948 results for: cloning

Showing: 141 - 160 of 51948 results

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CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#159281

Purpose

A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

AsCpf1-HA-2A-GFP

Use

Multicistronic vector with both caggs promoter-dr…

Tags

3X HA

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET mOrange LIC cloning vector (u-mOrange)

Plasmid

#29770

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mOrange

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET mCerulean LIC cloning vector (H6-mCerulean)

Plasmid

#29726

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin - His6 - TEV and mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (u-msfGFP)

Plasmid

#29772

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET Flag TEV LIC cloning vector (1L)

Plasmid

#29662

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

FLAG-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV LIC cloning vector (1R)

Plasmid

#29664

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Strep II-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-GFP-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pET mCerulean LIC cloning vector (u-mCerulean)

Plasmid

#29773

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET His10 TEV LIC cloning vector (2B-T-10)

Plasmid

#78173

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET mCitrine LIC cloning vector (u-mCitrine)

Plasmid

#29771

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mCitrine

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Use

Tags

Expression

Mutation

*

Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET PPL His6 LIC cloning vector (2J-T)

Plasmid

#37182

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and PPL

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET mRuby2 LIC cloning vector (u-mRuby2)

Plasmid

#86932

Purpose

pET LIC cloning vector for yORF-mRuby2

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

mRuby2

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET Flag TEV LIC cloning vector (2L-T)

Plasmid

#29715

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

FLAG-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV LIC cloning vector (2R-T)

Plasmid

#29717

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

StrepII-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

Plasmid

#139015

Purpose

To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Use

Tags

HA-MAO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

Plasmid

#132962

Purpose

Firefly-luciferase single-strand annealing assay cloning backbone

Depositor

Gang Bao

Article

Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

Insert

Firefly Luciferase

Use

CRISPR and Luciferase

Tags

Expression

Mammalian

Mutation

Stop sequence between luciferase gene

Promoter

CMV

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET mRuby2 LIC cloning vector (H6-mRuby2)

Plasmid

#86934

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

Biotin, His6, Tev Recognition Sequence, and mRuby…

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET tdTomato LIC cloning vector (H6-tdTomato)

Plasmid

#86928

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

Biotin, His6, Tev Recognition Sequence, and tdTom…

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET mClover LIC cloning vector (H6-mClover)

Plasmid

#86931

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-mClover

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

Biotin, His6, Tev Recognition Sequence, and mClov…

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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