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Showing: 141 - 160 of 320 results

  1. pJL1-sfGFP-MOORmut

    Plasmid
    #198213
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_MOORmut
    Use
    Tags
    6x His Tag and mutated (non-permissible) MOORmut …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    198213-plasmid-map-sequence-id-393674

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  2. Cx30-msfGFP

    Plasmid
    #69019
    Purpose
    Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx30 (GJB6 Human)
    Use
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    c
    Availability
    Academic Institutions and Nonprofits only
    69019-plasmid-map-sequence-id-127981

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  3. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

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  4. pRMC2-tat:msfGFP

    Plasmid
    #194915
    Purpose
    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    194915-plasmid-map-sequence-id-384626

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  5. pCAG-GFP-PQRv3-RFP

    Plasmid
    #73951
    Purpose
    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.
    Depositor
    Brian Chen
    Article
    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do
    Inserts
    sfGFP (superfolder GFP)
    RFP
    Use
    Tags
    A residual PQR peptide wii be left at the C-termi…
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin and Chicken beta actin (shared…
    Availability
    Academic Institutions and Nonprofits only

  8. pUS252b

    Plasmid
    #191831
    Purpose
    Expresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    fuGFPb
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    191831-plasmid-map-sequence-id-382128

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  9. pUS252L

    Plasmid
    #191832
    Purpose
    Expresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    fuBFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    191832-plasmid-map-sequence-id-382125

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  10. pUS252s

    Plasmid
    #191833
    Purpose
    Expresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    fuGFPs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    191833-plasmid-map-sequence-id-382124

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  11. pUS252y

    Plasmid
    #191834
    Purpose
    Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.
    Depositor
    Nicholas Coleman
    Insert
    fuYFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    191834-plasmid-map-sequence-id-382126

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  12. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Use
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    133451-plasmid-map-sequence-id-267048

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  14. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid
    #178043
    Purpose
    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP
    Depositor
    Han Xiao
    Article
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    178043-plasmid-map-sequence-id-359586

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  19. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid
    #141184
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has Service
    Cloning Grade DNA
    Insert
    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    Use
    Tags
    Signal peptide from influenza HA and Superfolder …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    141184-plasmid-map-sequence-id-306284

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  20. C3m

    Plasmid
    #120956
    Purpose
    MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)
    Depositor
    Richard Murray
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120956-plasmid-map-sequence-id-235970

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Showing: 141 - 160 of 320 results