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Addgene
Showing: 141 - 160 of 163 results
  1. II901: pMVP (L5-L4) FKBP DD

    Plasmid
    #121720
    Purpose
    pMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.
    Insert
    FKBP Degron Domain (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. II801: pMVP (L5-L4) ecDHFR DD

    Plasmid
    #121721
    Purpose
    pMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.
    Insert
    ecDHFR Degron Domain (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. KW701: pMVP (L5-L4) AID

    Plasmid
    #121723
    Purpose
    pMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.
    Insert
    AID (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid
    #121722
    Purpose
    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.
    Insert
    osTir1-P2A-AID (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    9x myc (C-term on osTIR1)
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121818
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121820
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 141 - 160 of 163 results