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Plasmid#207793PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.DepositorInsertsgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640a-1
Plasmid#42092DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 820-842 nt segment of ZEB1 mRNA (NM_…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-3
Plasmid#42091DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4565-4587 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-302s-1
Plasmid#42087DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4146-4168 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-215a-1
Plasmid#42085DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1259-1281 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-2
Plasmid#42090DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1956-1981 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-1
Plasmid#42089DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1419-1441 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-302a-1
Plasmid#42088DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4055-4078 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640a-2
Plasmid#42093DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1635-1657 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-215a-2
Plasmid#42086DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 2444-2466 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-MAPRE1
Plasmid#207794PurposeDonor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793DepositorInsertMAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-Solo-mScarlet-MAPRE1
Plasmid#227323PurposeDonor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)DepositorInsertMAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-MAPRE1
Plasmid#227324PurposeDonor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)DepositorInsertMAPRE1 Homology Arms flanking a mStayGold Tag (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pLuc-3'UTR
Plasmid#42094DepositorInsertZEB1 3UTR (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m215-2
Plasmid#42102DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-1+2
Plasmid#42106DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in two seed-comp…PromoterSV40Available sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-2
Plasmid#42105DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m215-1+2
Plasmid#42103DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in two seed-comp…PromoterSV40Available sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m640-1
Plasmid#42104DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only