Showing: 141 - 160 of 1885 results
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Plasmid#125564PurposeExpresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51(F86E)–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
hRad51(G151D)–Cas9(D10A)
Plasmid#125565PurposeExpresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51(G151D)–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
hRad51(K133R)–Cas9(D10A)
Plasmid#125568PurposeExpresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51(K133R)–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPD1019 TRE3GV DsRedExpress2 in TU2
Plasmid#139254PurposeTRE3GV promoter and DsRedExpress2 in TUPV2DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailabilityAcademic Institutions and Nonprofits only -
pPD1023 TRE3GV DsRedExpress2 in TU5
Plasmid#139257PurposeTRE3GV promoter and DsRedExpress2 in TUPV5DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailabilityAcademic Institutions and Nonprofits only -
pPD1026 EF1alpha TetON3G in TU2
Plasmid#139258PurposeExpresses TetON3G under hEF1alpha in TUPV2DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pPD1024 EF1alpha TetON3G in TU5
Plasmid#139261PurposeExpresses TetON3G under hEF1alpha in TUPV5DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pPD1025 EF1alpha TetON3G in TU6
Plasmid#139262PurposeExpresses TetON3G under hEF1alpha in TUPV6DepositorInsertTetON3G
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pPD107 VP16-ZF43(RARR)
Plasmid#138812PurposeConstitutive expression of VP16-ZF43(RARR) under the CMV promoter.DepositorInsertVP16-ZF43(RARR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPD108 VP16-ZF43(RAAR)
Plasmid#138813PurposeConstitutive expression of VP16-ZF43(RAAR) under the CMV promoter.DepositorInsertVP16-ZF43(RAAR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPD109 VP16-ZF43(RAAA)
Plasmid#138814PurposeConstitutive expression of VP16-ZF43(RAAA) under the CMV promoter.DepositorInsertVP16-ZF43(RAAA)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43A, R71APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 D102A, E108A
Plasmid#74618PurposeExpresses a double mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7AvailabilityAcademic Institutions and Nonprofits only -
AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA
Plasmid#199254PurposeExpression construct encoding the high specificity SpCas9-KARA-D10A nickaseDepositorInsertHigh specificity S. pyogenes Cas9-D10A-KARA nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848A R1060APromoterCAG promoterAvailabilityAcademic Institutions and Nonprofits only -
pX551-miniCMV-SpCas9D10A nickase
Plasmid#216735PurposeExpresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterT7 and mini-CMVAvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-SpCas9D10A nickase
Plasmid#216736PurposeExpresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterEF-1-alpha coreAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SpCas9D10A nickase
Plasmid#216737PurposeExpresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterCMVd1AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-G67M-invitro(KaganD100)
Plasmid#52014PurposeUsed for in vitro expression of the human MAVS CDS containing a point mutation G67MDepositorInsertMAVS
UseTagsExpressionMammalianMutationChanged Gly 67 to MetPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GFP-dynein-2(D1091–Q4307)
Plasmid#64064PurposeCodon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.DepositorInsertCytoplasmic dynein-2 (DYNC2H1 Human)
UseTagsGFP, His tag, and ZZ tagExpressionInsectMutationDeleted amino acids 1-1090, extra C-terminal vali…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pOTTC1124 - pAAV MeCP2 SpCas9(D10A)
Plasmid#112719PurposeAn AAV vector that expresses SpCas9 nickase under a neuronal cell promoterDepositorInsertSpCas9
UseAAVTagsExpressionMutationD10APromoterMecp2AvailabilityAcademic Institutions and Nonprofits only
Showing: 141 - 160 of 1885 results