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pSMAL-CellTag-multi-v1 barcode library

Pooled Library

#206045

Purpose

The CellTag multi library of barcodes can be used to clonally label cells with DNA barcodes. These barcodes can be profiled directly with single-cell RNA and ATAC sequencing, to allow clonal tracking

Depositor

Samantha Morris

Article

Jindal et al Nat Biotechnol. 2023 Sep 25. doi: 10.103

Use

Lentiviral

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only
Human Lentiviral sgRNA Library - Cell Cycle

Pooled Library

#51046

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for cell cycle-related targets.

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMT mir-1-1 reporter cel-mir-240

Plasmid

#46742

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-240

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter cel-mir-230

Plasmid

#46741

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-230

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Mutation

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-240

Plasmid

#46661

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-240

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-43-44 context

Plasmid

#46654

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-43-44 context

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-235

Plasmid

#46660

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-235

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-124

Plasmid

#46659

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-124

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Murine cell surface protein knockout (McspKO) library

Pooled Library

#200445

Purpose

A CRISPR library targeting 1,157 cell surface genes and 98 control genes with four sgRNAs per gene

Depositor

Junjie Chen

Article

Wang et al Proc Natl Acad Sci U S A. 2022 Jun 21;11

Expression

Mammalian

Species

Mus musculus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only
FNLCR CRISPRa Cell Surface and FNLCR CRISPRa Soluble Libraries

Pooled Library

#1000000228

Depositor

Raj Chari

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines

Pooled Library

#86538

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

Plasmid

#49069

Purpose

Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Article

Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

Insert

human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)

Use

Tags

3x Flag and mVenus

Expression

Mammalian

Mutation

C563S,C568S,C577S,C582S in hNKCC1 in NT17

Promoter

CMV

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

Plasmid

#49080

Purpose

Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Article

Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

Insert

human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)

Use

Tags

3x Flag and mVenus

Expression

Mammalian

Mutation

C791S, C820S, C1052S in hNKCC1 in NT17

Promoter

CMV

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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CRISPRi non-coding library (CRiNCL) - Common to all 5 cancer cell lines

Pooled Library

#86539

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV E

Plasmid

#145351

Purpose

Cell-free expression vector for Zika virus Envelope protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Envelope

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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CellTagA

Plasmid

#124591

Purpose

Expressed barcode for sample multiplexing in single-cell analysis

Depositor

Samantha Morris

Article

Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

Insert

CellTagA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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CellTagB

Plasmid

#124592

Purpose

Expressed barcode for sample multiplexing in single-cell analysis

Depositor

Samantha Morris

Article

Guo et al Genome Biol. 2019 May 9;20(1):90. doi: 1

Insert

CellTagB

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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dg263_pENTR_ChrimsonCel

Plasmid

#66101

Purpose

Entry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

Depositor

Dominique Glauser

Article

Schild et al Genetics. 2015 May 28. pii: genetics.115

Insert

Chrimson

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_eGFP_CELF1

Plasmid

#106100

Purpose

Inducible gene expression of EGFP - CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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