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Addgene
Showing: 1561 - 1580 of 32925 results
  1. pAL-pck_NT

    Plasmid
    #74069
    Purpose
    pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR
    Depositor
    Insert
    sgRNA pck (NT)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Availability
    Academic Institutions and Nonprofits only
  2. pAL-pyk_T

    Plasmid
    #74070
    Purpose
    pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR
    Depositor
    Insert
    sgRNA pyk (T)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Availability
    Academic Institutions and Nonprofits only
  3. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTA
    Depositor
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV-CMV-dSa VP64 Afp

    Plasmid
    #99697
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV
    Depositor
    Insert
    dCas9 and gRNA targeting Afp (Afp Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1561 - 1580 of 32925 results