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Plasmid#62923PurposeRetrovirus expression plasmidDepositorInsertIDH1 (IDH1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-Nono-sh5
Plasmid#127651PurposeKnock-down of human NONODepositorInsertNONO shRNA (NONO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H3C2
Plasmid#207783PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780DepositorArticleInsertH3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpCas9-Puro HLAG-2A-sgRNA
Plasmid#182551PurposeCas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2A-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIRIP-puro shRNA p19-1
Plasmid#14089DepositorInsertp19 ARF shRNA - 1 (Cdkn2a Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro Myr HA PIK3CB
Plasmid#12527DepositorInsertPIK3CB (PIK3CB Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationMyristoylation. Constitutively active allele.PromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC118-mCtnnb1-FLIP-Puro
Plasmid#84540PurposeCRISPR - FLIP targeting vector for mouse beta-cateninDepositorInsertmouse beta-catenin FLIP - Puro targeting vector
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ZIKVA-GFP-puro
Plasmid#140090PurposeFluorescence activatable reporter of ZIKV NS2B-NS3 protease activityDepositorInsertZIKVA-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIRIP-puro shRNA p19-2
Plasmid#14090DepositorInsertp19 ARF shRNA -2 (Cdkn2a Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-IFITM3g1 (BB15)
Plasmid#139460PurposeLentiviral vector with gRNA targeting IFITM3; includes puromycin selectable markerDepositorInsertIFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DENV2A-GFP-puro
Plasmid#140089PurposeFluorescence activatable reporter of DENV-2 NS2B-NS3 protease activityDepositorInsertDENV2A-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP5.v2 puro
Plasmid#87793PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorInsertshMKP5 (DUSP10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP5.v1 puro
Plasmid#87792PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorInsertshMKP5 (DUSP10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pf CAG mc2 IRES puro
Plasmid#206233PurposeFor expressing cloned exons as circular RNA.DepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBT/EFS-Cas9-PuroTk
Plasmid#170542PurposeExpresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinaseDepositorInsertPuroTK
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1#32-Tet-pLKO-puro
Plasmid#107399PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-TARDBP-A315T_VA
Plasmid#141329PurposeLentiviral vector for expression of C-terminally VA-tagged human TARDBP-A315T in mammalian cellsDepositorInsertTARDBP-A315T (TARDBP Human)
UseLentiviralTagsVA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)ExpressionMammalianMutationA315TPromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6sgbbBsmbI-puro-2A-Fluc
Plasmid#100277PurposeLentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imagingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg1 (CG509)
Plasmid#139455PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-A90V
Plasmid#133754PurposeLentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.DepositorInsertTARDBP (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged alanine 90 to valinePromoterCMVAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only