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Plasmid#97026PurposeyORFDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only
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pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector
Plasmid#139015PurposeTo express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targetingDepositorTypeEmpty backboneUseTagsHA-MAOExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-2xAPEX2-C Cloning Vector
Plasmid#136991PurposeTo express a protein of interest fused to the C-terminus of Flag-2xAPEX2 for proximity labeling or TEMDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOpen-SfiIR (cDNA)
Plasmid#165528PurposeType II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.DepositorInsertSfiI (used to create cDNA libraries)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag-APEX3-C Cloning Vector
Plasmid#220615PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)DepositorTypeEmpty backboneUseTagsFlag-APEX3 fusionExpressionMammalianMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
371-U
Plasmid#97036PurposeMYFQSNA-yORFDepositorTypeEmpty backboneUseTagsMYFQSNAExpressionBacterialMutationPromoterT7Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[Tc'hsp5'3'UTR]fa
Plasmid#86450PurposeHeat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)DepositorInsertTc'hsp5'3'UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-tag (T7) Vector
Plasmid#101137PurposeExpression of protein of interest with SNAP-Tag in bacterial cellsDepositorTypeEmpty backboneUseTagsSNAP-tag (SNAP26b)ExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.3G_X7
Plasmid#171213Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sitesDepositorInsertN/A; empty backbone
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mCherry LIC cloning vector (6B)
Plasmid#30125PurposeEmpty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-IRES-copGFP-T2A-Puro
Plasmid#72299PurposeExpress gene of interest under CB promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterchicken beta-actin + CMV enhancerAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-Spe4-Kozak-Snaptag-RfA
Plasmid#186385PurposeDestination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-emiRFP703-stop
Plasmid#186386PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mScarlet-I-stop
Plasmid#186393PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mScarlet-I into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-emiRFP703-RfA
Plasmid#186376PurposeDestination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mVenus-stop
Plasmid#186394PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-miRFP713-RfA
Plasmid#186381PurposeDestination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-Halotag-RfA
Plasmid#186377PurposeDestination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Snaptag-stop
Plasmid#186395PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only