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Plasmid#124791PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreenDepositorInsertmCherry-mNeonGreen
UsePcr templateTagsmCherry-mNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z32
Plasmid#120075PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-TagDepositorInsertS-Tag
UsePcr templateTagsS-TagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z30
Plasmid#120073PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-GDepositorInsertVSV-G
UsePcr templateTagsVSV-GExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z27
Plasmid#120070PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 1xHADepositorInsert1xHA
UsePcr templateTags1xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z24
Plasmid#120067PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAPDepositorInsertTAP
UsePcr templateTagsTAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z10
Plasmid#120053PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOkDepositorInsertmKOk
UsePcr templateTagsmKOkExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z01
Plasmid#120044PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P20
Plasmid#120031PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntagDepositorInsert10xSuntag
UsePcr templateTags10xSuntagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P16
Plasmid#120027PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3DepositorInsertDronpa3
UsePcr templateTagsDronpa3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CMV.Pro
Plasmid#79486PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoterDepositorInsertCMV Promoter
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCMV PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode9
Plasmid#83686Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode9
UseRetroviralTagsExpressionMutationplease view depositor comments belowPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode7
Plasmid#83684Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode7
UseRetroviralTagsExpressionMutationL119P in GFPPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCGS652
Plasmid#62220PurposeMammalian expression TALEN vector with N152, C+18 truncationsDepositorTypeEmpty backboneUseTALENTagsAcV5 and SV40 NLSExpressionMammalianMutationPromoterminiCaggsAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRDA_550
Plasmid#203398PurposeAll-in-one lentiviral expression of gRNA and EnAsCas12aDepositorInsertEnhanced Acidaminococcus Cas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-8
Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterP(rhaB)Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-iCas9-neo
Plasmid#85400PurposeLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequenceDepositorInsertFlag-iCas9-P2A-GFP
UseLentiviralTagsFlag-iCas9-P2A-GFPExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Accessory plasmid 1
Plasmid#128569Purposefor mixing with SPECS library to produce lentivirusDepositorInsertECFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only