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We narrowed to 13,574 results for: pET

Showing: 1541 - 1560 of 13574 results

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pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Tags

Expression

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-FnCas12a-TEV

Plasmid

#174658

Purpose

FnCas12a expression plasmid

Depositor

Huimin Zhao, Jin Wang

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

FnCas12a

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET30a-His6-LOXCAT

Plasmid

#140084

Purpose

Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

Depositor

Vamsi Mootha

Article

Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Insert

A fusion of lactate oxidase from A. viridans and catalase from E. coli

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET-15b-EK_C122S_His5

Plasmid

#49048

Purpose

Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

Depositor

Hans Brandstetter

Article

Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

Insert

Enteropeptidase, peptidase domain (TMPRSS15 Bovine)

Use

Tags

5xHis

Expression

Bacterial

Mutation

P92R, E185D, C122S

Promoter

T7

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3

Plasmid

#120288

Purpose

Produce human recombinant TGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)

Use

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-Cas9-His

Plasmid

#98158

Purpose

Purification of Cas9 protein

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

Insert

NLS-Cas9-NLS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Use

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a Halotag-CNA35

Plasmid

#131128

Purpose

Halotag-CNA35 peptide for Collagen labelling

Depositor

Samantha Stehbens

Article

Ju et al bioRxiv 2022

Insert

Halotag-CNA35

Use

Tags

Halotag

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Use

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-his-3C_Tau352

Plasmid

#100092

Purpose

Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

Depositor

Nikolai Sluchanko

Article

Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

Insert

Fetal microtubule-associated protein tau (MAPT Human)

Use

Tags

His6

Expression

Bacterial

Mutation

wild type sequence

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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p3990 pET ubcH8

Plasmid

#8650

Depositor

Peter Howley

Article

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

UbcH8 (UBE2L6 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Use

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Use

Tags

OneSTrEP-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-hTFIIB

Plasmid

#171084

Purpose

Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TF2B (GTF2B Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-21(+)Tm9D8* TrpB

Plasmid

#207517

Purpose

Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

Tryptophan synthase β-subunit

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL18

Plasmid

#183390

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET30a-AlkB-D135S

Plasmid

#79051

Purpose

Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Use

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET_RGG-GFP-RGG

Plasmid

#124948

Purpose

Expression of RGG-GFP-RGG

Depositor

Benjamin Schuster, Daniel Hammer, Matthew Good

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

EGFP and His

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b His-p97

Plasmid

#169020

Purpose

Expresses His-p97 in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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