-
Plasmid#109750PurposeUbiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertmKate2 fused to the actin binding lifeact sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2-HybridBarcode
Plasmid#174037PurposeFor Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sitesDepositorInsertCRISPR target sites
UseTol2 transgenesisTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pVk7-33G1-Flag
Plasmid#58950Purposebackbone for mouse recombinant antibody with added C-terminal FLAG tagDepositorTypeEmpty backboneUseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
sox10:PDGFRA wild-type
Plasmid#98696PurposeI-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafishDepositorInsertPDGFRA (PDGFRA Human)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-QF2w-SV40pA
Plasmid#155117PurposeMiddle entry vector containing QF2wDepositorInsertQF2w
UseGateway middle entry vectorTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
sox10:PDGFRA mutant
Plasmid#98697PurposeI-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafishDepositorInsertPDGFRA (PDGFRA Human)
UseZebrafish expressionTagsExpressionMutationexon 8 and 9 deletionPromoterAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
actc1b-Lifeact-mKate2
Plasmid#109493PurposeMuscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertactc1b-Lifeact-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bCE1-P1Egfp
Plasmid#90154Purposecontains cldn5b enhancer sequence, and cldn5b specific gene promoter driving expression of eGFPDepositorInsertcldn5bE1P1Egfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
Tol2-lyzC-microRNA 146a-Dendra2
Plasmid#97113Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmicroRNA 146a Dendra2
UseZebrafishTagsExpressionMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet28bZFCNGA5
Plasmid#69858PurposeHis tagged protein for antibody production screeningDepositorInsertZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab32b
Plasmid#109622PurposeMuscle specific zebrafish expression of mKate2 fused to rab32bDepositorInsertrab32b (rab32b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Park2_L (TAL 3012)
Plasmid#36004DepositorInsertTAL array targeting Park2 (prkn Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
Park2_R (TAL 3013)
Plasmid#36005DepositorInsertTAL array targeting Park2 (prkn Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
ptp4a3_L (TAL 3000)
Plasmid#35992DepositorInsertTAL array targeting ptp4a3 (ptp4a3a Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only