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Plasmid#149572Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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pBbdCas9H_arr2
Plasmid#149573Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_arr2
Plasmid#149575Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9B(RBSmut)_arr2
Plasmid#149579Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9H(RBSmut)_arr2
Plasmid#149580Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAftsI
Plasmid#149563PurposeftsI gRNA expression in B. burgdorferiDepositorInsertsgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAmreB
Plasmid#149567PurposemreB gRNA expression in B. burgdorferiDepositorInsertsgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
IX001: pBS/Fiber
Plasmid#131754PurposePlasmid containing Ad5 Fiber gene flanked by homology arms for insertion into the recombinant Ad5 vector IQ013 to generate tropism modified vectors.DepositorInsertAd5 wild-type Fiber
UseSynthetic BiologyTagsExpressionMutationPromoterN/AAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-dCas9_O
Plasmid#127028PurposeBasic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes.DepositorInsertPart dCas9_O
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-B0033-MV_RN
Plasmid#127016PurposeBasic Part RN- RBS+N-terminus; B0034m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0033m-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-RBS-pET-MV_RN
Plasmid#127014PurposeBasic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart RBS-pET-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
JK104: pMVP-DEST
Plasmid#121851PurposepMVP destination vector, empty expression plasmid backboneDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IH401: pMVP (L5-L4) CMV promoter
Plasmid#121726PurposepMVP L5-L4 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HE302: pMVP (L5-L4) RIP
Plasmid#121728PurposepMVP L5-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assemblyDepositorInsertRIP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO502: pMVP (R4-R3) eCFP
Plasmid#121734PurposepMVP R4-R3 entry plasmid, contains eCFP for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteCFP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO301: pMVP (R4-R3) eYFP
Plasmid#121736PurposepMVP R4-R3 entry plasmid, contains eYFP for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteYFP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HD301: pMVP (R4-R3) βgal
Plasmid#121738PurposepMVP R4-R3 entry plasmid, contains βgal for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertβgal
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HM001: pMVP (R4-R3) Cre
Plasmid#121740PurposepMVP R4-R3 entry plasmid, contains Cre for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertCre
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IY704: pMVP (L1-R5) EF1a promoter
Plasmid#121693PurposepMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP19-Tac
Plasmid#126958PurposeTerminator acceptor vector. Minimal cargo vector; with eGFP in downstream secondary site and constitutive promoter J23100 in upstream site. Allows introducing any part using BsmBI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only