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Plasmid#202011PurposeMoclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSPDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo level 1 vectorTagsExpressionMutationPromoterAtUBI10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-TAR C1
Plasmid#141246PurposeTransformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_HaloK73T_KDEL_pCMV1
Plasmid#183686PurposeMammalian expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)DepositorInsertER Halo(K73T)
UseTagsExpressionMammalianMutationK73T in HalotagPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C1
Plasmid#141248PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorInsertReduced Mitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-WDV-Rep-RepA-9
Plasmid#197716PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV Rep/RepA proteins: native ORFs
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-BeYDV-LS-L-11
Plasmid#197717PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV LSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-BeYDV-LRS-L-11
Plasmid#197718PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV LRSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-WDV-LS-L-11
Plasmid#197719PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV LSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-Cre-StLS1-9
Plasmid#197722PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCre recombinase with St-LS1 intron (maize optimized)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-RedFLuc-9
Plasmid#197738PurposePhytobrick (MoClo) Level 0 PartDepositorInsertRedF Luciferase (tomato optimized)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-TaGRF-GIF-9
Plasmid#197747PurposePhytobrick (MoClo) Level 0 PartDepositorInsertTaGRF-GIF chimeric protein CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-L2-Reverse-Donor-BsaI
Plasmid#197843PurposeDonor vector to restore L2 destination via BsaI (reverse orientation)DepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-L2-Reverse-Donor-Esp3I
Plasmid#197844PurposeDonor vector to restore L2 destination via Esp3I (reverse orientation)DepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-L1-Reverse-Donor-Esp3I
Plasmid#197845PurposeDonor vector to restore L1 destination via Esp3I (reverse orientation)DepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_L172Q_KDEL_pBABEpu
Plasmid#183690PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)DepositorInsertER Halo(L172Q)
UseRetroviralTagsExpressionMutationL72Q in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_M21K_F86L_KDEL_pBABEpu
Plasmid#183691PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)DepositorInsertER Halo(M21K_F86L)
UseRetroviralTagsExpressionMutationM21K_F86L in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_Jdomain
Plasmid#183693PurposeBacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domainDepositorInsertER Halo(K73T)-J domain
UseTagsExpressionBacterialMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-CteIF4E_35-250-del183-202_AF
Plasmid#148721PurposeBacterial Expression of CteIF4E_35-250-del183-202DepositorInsertCteIF4E_35-250-del183-202
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-CteIF4E_40-250-del183-202_AF
Plasmid#148723PurposeBacterial Expression of CteIF4E_40-250-del183-202DepositorInsertCteIF4E_40-250-del183-202
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-CteIF4E_35-250-del183-202_AF
Plasmid#148725PurposeBacterial Expression of CteIF4E_35-250-del183-202DepositorInsertCteIF4E_35-250-del183-202
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only