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Plasmid#162794PurposeCcnb2 shRNA in pMKO.1 retroviral vectorDepositorInsertCcnb2 shRNA (Ccnb2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EIF2S3_GTP-EFTU
Plasmid#109829PurposeProtein expression and purification of EIF2S3_GTP-EFTUDepositorInsertEIF2S3_GTP-EFTU (EIF2S3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EFS-1/1
Plasmid#91456PurposeProtein expression and purification of human SH3 domain construct EFS-1/1DepositorInsertEFS-1/1 (EFS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pIS58 TIP120A 3'UTR mut
Plasmid#14502DepositorInsertTIP120A 3'UTR mutant (CAND1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCC’s were changed to AAGTAC'sPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP wildtype
Plasmid#73048PurposeC-terminal fusion of gfp Mouse wildtype Foxg1DepositorInsertmFoxg1 (Foxg1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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GPR83-Tango
Plasmid#66376PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR83 (GPR83 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2
Plasmid#89308Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.DepositorInsertMS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA
Plasmid#218528PurposesgRNA targeting human EIF2AK4/GCN2DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF.STAT3DN.Ubc.GFP
Plasmid#24984PurposeDepositorInsertSTAT3 (STAT3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationY705F--dominant negativePromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
ACTN2-pEGFP
Plasmid#52669PurposeOverexpression of GFP-tagged α-actinin-2DepositorInsertactinin 2 (ACTN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF_PE2
Plasmid#199267PurposeMammalian expression of SpCas9 prime editor 2 under EF1α promoterDepositorInsertPE2-P2A-mp53DD
UseTagsExpressionMammalianMutationΔ40-903PromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pBabe-puro
Plasmid#19005DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pcDNA3
Plasmid#18955DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
Plasmid#173862PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)DepositorInsertAktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-P405A/P531A-pcDNA3
Plasmid#18956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pP{ELAV-GeneSwitch}
Plasmid#83957PurposeExpresses conditional RU486-dependent GAL4 protein under control of the elav promotorDepositorInsertUseTagsExpressionInsectMutationPromoterelavAvailable sinceDec. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-EF1a-CreERT2-3Xflag-T2A-eBFP2
Plasmid#170186PurposeThis Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracingDepositorInsertCreERT2-T2A-eBFP2
UseLentiviralTagsExpressionMutationPromoterEF1 alphaAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only