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Plasmid#162373PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8f
UseTags6xHisExpressionMammalianMutationQ315LPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRESTO-Tango Kit
Plasmid Kit#1000000068PurposeUsed to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.DepositorAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Puro (658-5)
Plasmid#17448Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, PuroDepositorHas ServiceCloning Grade DNAInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE (AAV1)
Viral Prep#104488-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-Ubiquitin
Plasmid#18712PurposeMammalian expression of human ubiquitin with HA tagDepositorInsertubiquitin (UBB Human)
UseTagsHAExpressionMammalianMutationThe HA-Ubiquitin insert is from the UBB gene. Theā¦PromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_Grx1_roGFP2
Plasmid#155045PurposeFluorescent redox reporterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby (AAV1)
Viral Prep#71760-AAV1PurposeReady-to-use AAV1 particles produced from pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby (#71760). In addition to the viral particles, you will also receive purified pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby plasmid DNA. Syn-driven, Cre-dependent expression of membrane-bound GFP and the presynaptic protein synaptophysin fused to mRuby. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmGFP, mRuby (Cre-dependent)Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseTagsExpressionBacterialMutationPromoternative promoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-I-SceI
Plasmid#60960PurposePlasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesisDepositorInsertI-SceI restriction enzyme
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-R-Creon shRNA[Control]
Plasmid#180775PurposeThis vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.DepositorTypeEmpty backboneUseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only