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pcDNA5FRT/Flag-hINO80C (1224-1556)

Plasmid

#29445

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 1224-1556 (a.a.)

Promoter

Available since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
CCM2(FL)_pET49b

Plasmid

#224066

Purpose

Bacterial expression of full-length CCM2 fused to an N-terminal GST. HRV-3C cleavage site

Depositor

Ben Goult

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

ccm2 (CCM2 Human)

Use

Tags

GST; 6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CCM2_d410-444_eGFP

Plasmid

#208877

Purpose

Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Neil J. et al BioRxiv

Insert

CCM2 (CCM2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

amino acids deletion : 410-444

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-4xmut-dsRNAres_R

Plasmid

#147425

Purpose

Insect Expression of DmCCR4-4xmut-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-4xmut-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_1-166-L42EI44E-dsRNAres_R

Plasmid

#147426

Purpose

Insect Expression of DmCCR4_1-166-L42EI44E-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_1-166-L42EI44E-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-L42EI44E-dsRNAres_R

Plasmid

#147429

Purpose

Insect Expression of DmCCR4-L42EI44E-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-L42EI44E-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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CEA-N domain residues 1-132 (CEACAM5)-pET30b

Plasmid

#174693

Purpose

Plasmid expressing CEA-N domain

Depositor

Jean Gariepy

Article

Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

Insert

CEA-N Domain

Use

Tags

TEV Cleavage Site and hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mGas8-PA

Plasmid

#176468

Purpose

Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

growth arrest specific 8 (Gas8 Mouse)

Use

Tags

PA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-33/myc-His

Plasmid

#127492

Purpose

Expresses myc-tagged _MTS NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-33 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-33

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _2-78/myc-His

Plasmid

#127493

Purpose

Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-78 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-78

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Article

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-ORF9B

Plasmid

#154425

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF9B

Use

Gateway-compatible entry vector, with insert of o…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 S_nostop

Plasmid

#152988

Purpose

Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

Plasmid

#154029

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 ORF3B

Plasmid

#141272

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207 SARS-CoV-2 NSP3 Cys857Ala

Plasmid

#154027

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PC4_3spc34h

Plasmid

#70700

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Use

Tags

his-tag on C-terminus of spc34

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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PC4_3dad1h

Plasmid

#70699

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on dad1

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Use

Tags

his-tag on C-terminus of dad1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 N_nostop

Plasmid

#152987

Purpose

Gateway-compatible Entry vector, with insert of N gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

N

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-E

Plasmid

#154418

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

E (E SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of e…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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