Addgene

We narrowed to 2,594 results for: BFP

Showing: 1501 - 1520 of 2594 results

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pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHRm-NLS-dCas9-GFP11x7-NLS

Plasmid

#70224

Purpose

Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

NLS-dCas9-GFP11x7-NLS

Use

Tags

GFP11x7

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAN2981

Plasmid

#127246

Purpose

Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgULK1-1

Plasmid

#109004

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ULK1-sgRNA #1 (ULK1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAN1579

Plasmid

#127201

Purpose

Progesterone inducible expression of KeyC-BFP

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pPAB1-Z3PM-tTDH1-pZ3-key_C-mTagBFP2-tENO1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAN1572

Plasmid

#127202

Purpose

Dual inducible expression of Venus-degronSwitchA and KeyA-BFP

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-2

Plasmid

#109009

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #2 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEIF3D-a11helixDel-sgEIF3D

Plasmid

#222135

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Expression

Mutation

T317E, N320E, H321A

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-CtermDel-sgEIF3D

Plasmid

#222125

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Expression

Mutation

F527 truncation

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-a5helixDel-sgEIF3D

Plasmid

#222133

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Expression

Mutation

D249Q, V262I, Y263A

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528N-S529N-sgEIF3D

Plasmid

#222129

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Expression

Mutation

S528N, S529N

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00029 (aka pRC0610)

Plasmid

#200834

Purpose

Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1-RNF169UBD

Plasmid

#119009

Purpose

Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1-Rad18UBD

Plasmid

#119008

Purpose

Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MTK7_010

Plasmid

#123877

Purpose

Encodes promoter-less tagBFP T2A Blasticidin resistance cassette to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BFP-T2A-Blasticidin-BgPa

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAN1578

Plasmid

#127200

Purpose

Progesterone inducible expression of KeyA-BFP

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pPAB1-Z3PM-tTDH1-pZ3-key_A_e18-mTagBFP2-tENO1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pBA439

Plasmid

#85967

Purpose

Perturb-seq vector backbone

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

Plasmid

#192939

Purpose

FlpO-based digitizer circuit driven by AP1 promoter

Depositor

Wilson Wong

Article

Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:

Inserts

AP1(2)/minTK promoter
FlpO-ABI
PYL-FlpO
CAG promoter
frt-STOP-frt
GFP
luciferase
pPGK
Zeocin resistance
BFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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