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pMSR:MSMEG_5048:Dendra

Plasmid

#167366

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_5048

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0028:Dendra

Plasmid

#159038

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0028

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Fzd6

Plasmid

#123588

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

frizzled class receptor 6 (Fzd6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

Plasmid

#135565

Purpose

An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

miR-30 rat SST
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

SYN1 and hSYN1

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_01

Plasmid

#158102

Purpose

5'UTR stem-loop luciferase reporter: 0kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop 0kcal/mol

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RR

Plasmid

#108862

Purpose

FnCpf1 Gateway entry plasmid with N623R and K687R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RR (FnCpf1 with N623R and K687R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R and K687R, Cpf1 is rice codon optimized

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Use

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0132:Dendra

Plasmid

#159002

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0132

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_02

Plasmid

#158103

Purpose

5'UTR stem-loop luciferase reporter: -30kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop -30kcal/mol

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0319:Dendra

Plasmid

#159010

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0319

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Mutation

Promoter

pmycTetO

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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