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Plasmid#40639DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…ExpressionBacterialMutationS235APromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBEL1954
Plasmid#195689PurposeExpression/purification of 6xHis-TEV-MlaD(F118A/L123A)DepositorInsertMlaD
UseTagsExpressionBacterialMutation6xHis-TEV-MlaD(F118A/L123A)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2707
Plasmid#195636PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA (227-251)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2753
Plasmid#195637PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA (227-251; I241N)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2754
Plasmid#195638PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA (227-251; L245N)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2755
Plasmid#195639PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA (227-251; I248N)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2756
Plasmid#195640PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2757
Plasmid#195641PurposeExpression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)DepositorInsertsfGFP-MlaA
UseTagsExpressionBacterialMutation6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)PromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pThuAsol
Plasmid#171675PurposepThuAsol expression in E. coliDepositorInsertSUMO-TEV-ThuAsol
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDB367
Plasmid#110277PurposegHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusionDepositorInsertgHEEE_02
UseTags10-His, Tev recognition peptide, and mature DsbC …ExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-F219L (JBEI-7186)
Plasmid#110151PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-F219L mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_Gateway-3xFLAG
Plasmid#172981PurposeGateway Destination vector for doxycycline-inducible expression of ORFDepositorTypeEmpty backboneUseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
Plasmid#81185PurposeExpresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5cDepositorInsertsCas5c
Cas8c
Cas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertDNPH1 (DNPH1 Human)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAP2050-2
Plasmid#105243Purposemammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linkerDepositorInsertGFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP2042-1
Plasmid#105244Purposemammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linkerDepositorInserteGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSD118A
Plasmid#218140PurposeExpresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coliDepositorInsertSD0_ZF2-3
UseTagsAviTag-6xHis-MBP-TEV-(GGGGS)2ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:S495A
Plasmid#98151PurposeExpresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:S495A
UseTagsHis6-TEVExpressionBacterialMutationChanged serine 495 to alaninePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:W603A
Plasmid#98153PurposeExpresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:W603A
UseTagsHis6-TEVExpressionBacterialMutationChanged tryptophan 603 to alaninePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only