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Plasmid#185786PurposeEncodes enhanced green fluorescent protein linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_EGFP-P2A_truncHaus6_IRES_Blast
Plasmid#182885PurposeTransfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.DepositorInsertHAUS 6 (HAUS6 Human)
UseLentiviralTagsExpressionBacterial and MammalianMutationstop codon introduced 2 amino acids downstream of…PromoterEF1alpha coreAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-(L)-mApple
Plasmid#182856PurposeHetero-dimer expression vectorDepositorInsertmEGFP-mApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-HDEL
Plasmid#136097PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptideDepositorInsertCTAG_eGFP(noATG,noStop)-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CT-Tagging
Plasmid#165871PurposeTemplate for amplification of EGFP C-terminal tagging cassetteDepositorInsertVector - CT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.mArid3a-3'UTR-WT
Plasmid#169299PurposeConstitutive overexpression of the 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.DepositorInsertArid3a-3'UTR-WT (Arid3a Mouse)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NT-Tagging
Plasmid#165868PurposeTemplate for amplification of EGFP N-terminal tagging cassetteDepositorInsertVector - NT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TL1
Plasmid#165834PurposesfGFP green fluorescent protein for N-terminal taggingDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TL5
Plasmid#165835PurposesfGFP green fluorescent protein for C-terminal taggingDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH–PB
Plasmid#169685PurposeAn anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody
UseTagsmodified STIM1-PB tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.ARID3A-3'UTR-WT
Plasmid#169297PurposeConstitutive overexpression of the 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.DepositorInsertARID3A-3'UTR-WT (ARID3A Human)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 AURKA 1-30
Plasmid#157756PurposeExpression of AURKA Nter (aa1-30):GFP in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionMammalianMutationThe first 30 amino acids of AURKA were clonedPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(2)
Plasmid#163428PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19CYT
Plasmid#155248Purposefor mammalian overexpression of the CYT isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (Cytoplasmic isoform) (Usp19 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-rUSP19ER
Plasmid#155247Purposefor mammalian overexpression of the ER isoform of rat USP19 fused to GFP at N-terminusDepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRTagsExpressionMammalianMutationPromoterNoneAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pStrep-5xEGFP-N1
Plasmid#122492PurposeExpression of Strep-tagged 5xEGFPDepositorInsertStrep-tagged 5xEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only