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We narrowed to 1,681 results for: rpe

Showing: 1461 - 1480 of 1681 results

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pQCXIP mScarlet-MOSPD2 RD/LD

Plasmid

#186476

Purpose

Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Chrimson-GFP

Plasmid

#62718

Purpose

AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

Plasmid

#217893

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Beatrix

Plasmid

#167528

Purpose

Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

Depositor

Gian Michele Ratto

Article

Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

Insert

Beatrix

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-rtTAV16

Plasmid

#102367

Purpose

AAV vector to drive TET-ON activator expression from human synapsin I promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

Insert

rtTAV16

Use

AAV

Tags

Expression

Mammalian

Mutation

rtTA variant reported in Gene Therapy (2006) 13, …

Promoter

human synapsin I promoter

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-fwd[Chrimson-tdT]

Plasmid

#59137

Purpose

Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson-tdTomato

Use

Cre/Lox

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-GFP

Plasmid

#122100

Purpose

AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

GFP

Use

AAV

Tags

NA

Expression

Mammalian

Mutation

Promoter

EF1a（1.1kb short version)

Available since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-ER [TM(SAC1)]

Plasmid

#186572

Purpose

Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG 17xS/T->A, catalytic active lipin 1

Plasmid

#32007

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

Promoter

CMV, SP6

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-Chronos-GFP

Plasmid

#99232

Purpose

AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

Plasmid

#32008

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

Promoter

CMV, SP6

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pET51b-Strep-hAGT-His

Plasmid

#167276

Purpose

Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

hAGT (AGT Human)

Use

Tags

His x10 and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Herpes Simplex Virus protein VP16, Budding Yeast, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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FB013

Plasmid

#119710

Purpose

TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Tags

Expression

Mutation

V30M

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAGT9569

Plasmid

#219431

Purpose

Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Tags

PapE-4LF2

Expression

Plant

Mutation

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN002P-M-FLAG-His

Plasmid

#218026

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN002P-M

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN002X-N-FLAG-His

Plasmid

#218033

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN002X-N

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN002W-V-FLAG-His

Plasmid

#218024

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN002W-V

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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