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Plasmid#149578Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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pX28
Plasmid#46850PurposeTOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiencyDepositorInsertattB, RFP and PGK-puro
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9K(-10TC)_arr2
Plasmid#149584Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9K_arr2
Plasmid#149570Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9K(RBSmut)_arr2
Plasmid#149577Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT3
Plasmid#79500PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3 CDSDepositorInsertWNT3 (WNT3 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Oncostatin M toehold switch sensor
Plasmid#111909PurposeToehold switch sensor to detect Oncostatin M mRNA with GFP outputDepositorInsertOncostatin M toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P21
Plasmid#120032PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTagDepositorInsert24xSunTag
UsePcr templateTags24xSunTagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z04
Plasmid#120047PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1DepositorInsertmTFP1
UsePcr templateTagsmTFP1ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P30
Plasmid#120041PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-GDepositorInsertVSV-G
UsePcr templateTagsVSV-GExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-cat_TU
Plasmid#127051PurposeBasic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.DepositorInsertPart Cat_TU
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-B0032-MV_RN
Plasmid#127015PurposeBasic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.DepositorInsertPart B0032m-MV_RN
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP19-min2
Plasmid#126956Purpose"Minimal cargo" vector. Allows introducing any part using BsmBI; but doesn't have cloning reporter. Can be used to generate new promoter/terminator acceptor vectors.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-25
Plasmid#120004PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LIDDepositorInsertYFP-LID
UsePcr templateTagsYFP-LIDExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-19
Plasmid#124787PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreenDepositorInsertmCherry-mNeonGreen
UsePcr templateTagsmCherry-mNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG703: pMVP (L1-R5) TRE promoter
Plasmid#121697PurposepMVP L1-R5 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTRE promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD401
Plasmid#91828PurposePmyo-2::GFP FP-slot donor for the SapTrap cloning systemDepositorInsertPmyo-2::GFP
UseCRISPRTagsExpressionWormMutationNucleotide 489 of the myo-2 promoter was changed …PromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P03
Plasmid#120014PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3DepositorInsertmoxCerulean3
UsePcr templateTagsmoxCerulean3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT3A
Plasmid#79501PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDSDepositorInsertWNT3A (WNT3A Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -