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Plasmid#140199PurposeEpisomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.DepositorInsertdSpCas9-VPR
UseCRISPR and Synthetic Biology; Expression in asper…TagsVPR (VP64-p65-Rta), NLSExpressionMutationPromoterPgpdAAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE
Plasmid#123360PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.DepositorInsertEmpty LbCpf1 crRNA cloning cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC
Plasmid#140197PurposeEpisomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.DepositorInsertdLbCas12a-VPR
UseCRISPR and Synthetic Biology; Expression in asper…TagsVPR (VP64-p65-Rta), NLSExpressionMutationD832A DNase deactivatedPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p213-SWITCH-OFF
Plasmid#217888PurposeRetroviral Switch-OFF vector for sgRNA expression; U6-BbsIx2-SWITCH-OFF-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p205-5'-Switch-ON
Plasmid#217884PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p204-U6-Switch-ON
Plasmid#217883PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK880-pCAG-DmrC-NLS-3xFLAG-VP64
Plasmid#136912PurposeDmrC with VP64 fused to it's C-terminus; Mammalian expression vectorDepositorInsertpCAG-DmrC-NLS-3xFLAG-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterChicken Beta ActinAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SpCas9
Plasmid#104588PurposeAAV vector expressing Myc-tagged SpCas9 under the EFS promoterDepositorInsertStreptococcus pyogenes Cas9
UseAAV and CRISPRTagsMycExpressionMammalianMutationPromoterEFSAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_gRNA
Plasmid#80457PurposegRNA expression vector with mCherry transfection controlDepositorInsertssp Cas9 gRNA
mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and chicken β-actin promoterAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-KRAB
Plasmid#50919PurposeConstitutive dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-VP64
Plasmid#50918PurposeConstitutive dCas9-VP64 lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGH335_MS2-AID*Δ-Hygro
Plasmid#85406Purposelentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable markerDepositorInsertMS2-AID*Δ and Hygromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ccas9LacZ
Plasmid#113083PurposeEnhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIC-Cas9
Plasmid#121657Purposegateway cloning destination vector for expressing enhancer driven Cas9DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV_A2S-8
Plasmid#188654PurposeAAV vector expressing SaAID-2S and gRNADepositorInsertsAID2S--nSaCas9-UGI
Sa-sgRNA
UseAAV and CRISPRTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterScp1 and U6Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSARN-vasa-eSpCas9sv40
Plasmid#173670PurposeeSpCas9 expression vector for AnophelesDepositorInserteSpCas9 with Anopheles vasa promoter and SV40 terminator
UseCRISPRTagsExpressionInsectMutationmutations in Cas9 reducing off-target activity (S…PromotervasaAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-U61-SapI
Plasmid#112808PurposegRNA cloning vector for expressing 1-3 gRNAs in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-mRxxFP1(AfeI)-iP-A
Plasmid#204625PurposeCloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AA368
Plasmid#215972PurposeFragmid fragment: (Pol 2 promoter) ubiquitous expressionDepositorHas ServiceCloning Grade DNAInsertAct5C_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only