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Showing: 1461 - 1480 of 1983 results

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  4. Ypq2-2GFP-URA

    Plasmid
    #207010
    Purpose
    Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    YPQ2
    Use
    Tags
    2xGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207010-plasmid-map-sequence-id-422256

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  5. Vph1-2GFP-URA

    Plasmid
    #207009
    Purpose
    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    VPH1
    Use
    Tags
    2xGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207009-plasmid-map-sequence-id-422255

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  10. ePPEmax*

    Plasmid
    #205244
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    205244-plasmid-map-sequence-id-422881

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  11. eSrtA-MT4

    Plasmid
    #200306
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT4 (MT4 Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200306-plasmid-map-sequence-id-394863

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  12. SUMO_LPETG_MT3

    Plasmid
    #200304
    Purpose
    Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200304-plasmid-map-sequence-id-394896

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  13. SUMO_MT3

    Plasmid
    #200303
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200303-plasmid-map-sequence-id-394864

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  14. pTC3cIfm

    Plasmid
    #193778
    Purpose
    Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193778-plasmid-map-sequence-id-389809

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  18. pMM4_14

    Plasmid
    #183062
    Purpose
    Plasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.
    Inserts
    mCherry
    BM3R1-HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HREsYGP1p and RET2
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183062-plasmid-map-sequence-id-363407

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  20. pLBM0040

    Plasmid
    #172819
    Purpose
    Level 2 module containing HSP21-nYFP deltaPTAC5-cYFP
    Depositor
    Benjamin Field
    Article
    Velay et al Plant Methods. 2022 May 26;18(1):69. doi
    Insert
    [35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-deltamPTAC5-cYFPX3HA][35S-SP_SV40-CFP]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    172819-plasmid-map-sequence-id-348677

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Showing: 1461 - 1480 of 1983 results